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Mfg Part Number

Ab03705-10.3

Vector Labs Ab03705-10.3 Anti-Sclerostin [AbD09097], Human IgG1, Fc Silent™, kappa (100 μg)

VECTOR LABS

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Quick Overview

The original antibody was isolated from the HuCAL GOLD library by panning against mouse sclerostin protein biotinylated using EZ-link Sulfo-NHS-LC-Biotin and coupled to streptavidin-coated magnetic beads.

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Description

Product Class: Purified
Clone ID: AbD09097
Heavy Chain Modification: Fc Silent™
Synonyms: SOST
Amount: 100 μg
Application Codes Clone: ELISA; neutralize
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: Fab
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This reformatted human antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds mouse sclerostin and cross reacts with human sclerostin and recognizes an epitope adjacent to sclerostin's binding site for the Wnt co-receptor LRP6. Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain which is produced in osteocytes (type of bone cell). The glycoprotein sclerostin has been identified as a negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation. Neutralizing anti-sclerostin antibodies are able to restore Wnt activity and enhance bone growth thereby presenting a new osteoanabolic therapy approach for diseases such as osteoporosis.
Application Notes: The binding reactivity of this antibody for mouse sclerostin was tested using ELISA. This antibody binds mouse and human sclerostin with a binding affinity of Kd= 46nM and Kd=260 nM respectively. This antibody efficiently neutralizes sclerostin's Wnt inhibitory activity. In vitro interaction analysis using sclerostin variants revealed that this neutralizing Fab binds to sclerostin's flexible second loop, which has been shown to harbor the LRP5/6 binding motif (PMID: 27558933).

Product Class:	Purified
Clone ID:	AbD09097
Heavy Chain Modification:	Fc Silent™
Synonyms:	SOST
Amount:	100 μg
Application Codes Clone:	ELISA; neutralize
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	Fab
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This reformatted human antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds mouse sclerostin and cross reacts with human sclerostin and recognizes an epitope adjacent to sclerostin's binding site for the Wnt co-receptor LRP6. Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain which is produced in osteocytes (type of bone cell). The glycoprotein sclerostin has been identified as a negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation. Neutralizing anti-sclerostin antibodies are able to restore Wnt activity and enhance bone growth thereby presenting a new osteoanabolic therapy approach for diseases such as osteoporosis.
Application Notes:	The binding reactivity of this antibody for mouse sclerostin was tested using ELISA. This antibody binds mouse and human sclerostin with a binding affinity of Kd= 46nM and Kd=260 nM respectively. This antibody efficiently neutralizes sclerostin's Wnt inhibitory activity. In vitro interaction analysis using sclerostin variants revealed that this neutralizing Fab binds to sclerostin's flexible second loop, which has been shown to harbor the LRP5/6 binding motif (PMID: 27558933).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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