Vector Labs Ab03729-1.1 Anti-E2 [10B5 (RS10B5huFc)], Mouse IgG1, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 10B5 (RS10B5huFc)
Synonyms: WEEV E2; Envelope glycoprotein; E2 envelope glycoprotein; Structural polyprotein; Spike glycoprotein E2; p130; Alphavirus; 10B5 E7E2
Amount: 100 μg
Application Codes Clone: ELISA
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds the E2 envelope polyprotein of the Western equine encephalitis virus (WEEV), which is a mosquito-borne arbovirus (arthropod-borne virus) and the causative agent of western equine encephalitis (WEE). WEEV belongs to the genus Alphavirus in the family Togaviridae. The virus is transmitted to people and horses by bites from infected mosquitoes (Culex tarsalis and Aedes taeniorhynchus) and birds during wet, summer months. Infections of humans and horses can be fatal, and survivors often suffer permanent neurological sequelae. Most of the infections are subclinical, presenting as fever, chills, malaise, and myalgias, but some can progress to an acute inflammation of the meninges and brain parenchyma.
Application Notes: This antibody can bind the WEEV E2 envelope glycoprotein in an ELISA. The human Fc domain of the antibody was capable of effector functions, such as binding to protein G and human complement (PMID: 10768836). This antibody was found to possess a binding affinity to a WEE virus epitope (K[D] = 9.14 × (10)^-6 M). This antibody was also reported to not cross react with other alphaviruses like Sindbis virus [SIN], Venezuelan equine encephalitis [VEE] virus, and eastern equine encephalitis [EEE] virus. Pharmacokinetics studies showed that the RS10B5huFc antibody (free and encapsulated) was found to be retained in the lungs of mice for greater than 48 h when administered intranasally. In contrast, when administered intramuscularly to mice, the RS10B5huFc antibody was not detected in the lungs and only found in the liver and kidneys (PMID: 11289221).

Product Class:	Purified
Clone ID:	10B5 (RS10B5huFc)
Synonyms:	WEEV E2; Envelope glycoprotein; E2 envelope glycoprotein; Structural polyprotein; Spike glycoprotein E2; p130; Alphavirus; 10B5 E7E2
Amount:	100 μg
Application Codes Clone:	ELISA
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds the E2 envelope polyprotein of the Western equine encephalitis virus (WEEV), which is a mosquito-borne arbovirus (arthropod-borne virus) and the causative agent of western equine encephalitis (WEE). WEEV belongs to the genus Alphavirus in the family Togaviridae. The virus is transmitted to people and horses by bites from infected mosquitoes (Culex tarsalis and Aedes taeniorhynchus) and birds during wet, summer months. Infections of humans and horses can be fatal, and survivors often suffer permanent neurological sequelae. Most of the infections are subclinical, presenting as fever, chills, malaise, and myalgias, but some can progress to an acute inflammation of the meninges and brain parenchyma.
Application Notes:	This antibody can bind the WEEV E2 envelope glycoprotein in an ELISA. The human Fc domain of the antibody was capable of effector functions, such as binding to protein G and human complement (PMID: 10768836). This antibody was found to possess a binding affinity to a WEE virus epitope (K[D] = 9.14 × (10)^-6 M). This antibody was also reported to not cross react with other alphaviruses like Sindbis virus [SIN], Venezuelan equine encephalitis [VEE] virus, and eastern equine encephalitis [EEE] virus. Pharmacokinetics studies showed that the RS10B5huFc antibody (free and encapsulated) was found to be retained in the lungs of mice for greater than 48 h when administered intranasally. In contrast, when administered intramuscularly to mice, the RS10B5huFc antibody was not detected in the lungs and only found in the liver and kidneys (PMID: 11289221).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

Reviews