Vector Labs Ab03734-21.0 Anti-E1 protein [DC1.7], Mouse IgM, kappa (50 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: DC1.7
Synonyms: CHIKV E1; Structural polyprotein; Envelope glycoprotein E1; Spike glycoprotein E1
Amount: 50 μg
Application Codes Clone: ELISA; BLI; FC; IP; neutralize
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds and epitope near the E1 DIII domain of the Chikungunya virus (CHIKV), which is the causative agent of zoonotic disease in humans called Chikungunya. Additionally, this antibody also cross reacts with multiple arthritogenic alphaviruses like Mayaro virus (MAYV), Ross River (RRV) and Sindbis virus (SINV) (PMID: 34416146). CHIKV belongs to the alphavirus genus of the Togaviridae family and is transmitted to human by infected mosquito bites. A CHIKV infection causes fever and severe joint pain. Other symptoms include muscle pain, joint swelling, headache, nausea, fatigue and rash. The disease mostly occurs in Africa, Asia and the Indian subcontinent.
Application Notes: The binding characterization of the IgG antibody for CHIKV E1 protein was done using ELISA and Biolayer interferometry (BLI). This antibody was capable of binding biotinylated p62-E1 hybrid protein in a flow cytometric assay. This antibody was also capable of immunoprecipitating the E1 envelope protein of CHIKV. This antibody was can neutralize CHIKV 181/25 and CHIKV LR2006_OPY1 with IC50 value in the low nanomolar range and 13nM respectively. This antibody could not provide sufficient in vivo protection from CHIKV infection (PMID: 31697791).

Product Class:	Purified
Clone ID:	DC1.7
Synonyms:	CHIKV E1; Structural polyprotein; Envelope glycoprotein E1; Spike glycoprotein E1
Amount:	50 μg
Application Codes Clone:	ELISA; BLI; FC; IP; neutralize
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds and epitope near the E1 DIII domain of the Chikungunya virus (CHIKV), which is the causative agent of zoonotic disease in humans called Chikungunya. Additionally, this antibody also cross reacts with multiple arthritogenic alphaviruses like Mayaro virus (MAYV), Ross River (RRV) and Sindbis virus (SINV) (PMID: 34416146). CHIKV belongs to the alphavirus genus of the Togaviridae family and is transmitted to human by infected mosquito bites. A CHIKV infection causes fever and severe joint pain. Other symptoms include muscle pain, joint swelling, headache, nausea, fatigue and rash. The disease mostly occurs in Africa, Asia and the Indian subcontinent.
Application Notes:	The binding characterization of the IgG antibody for CHIKV E1 protein was done using ELISA and Biolayer interferometry (BLI). This antibody was capable of binding biotinylated p62-E1 hybrid protein in a flow cytometric assay. This antibody was also capable of immunoprecipitating the E1 envelope protein of CHIKV. This antibody was can neutralize CHIKV 181/25 and CHIKV LR2006_OPY1 with IC50 value in the low nanomolar range and 13nM respectively. This antibody could not provide sufficient in vivo protection from CHIKV infection (PMID: 31697791).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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