Vector Labs Ab03738-10.3 Anti-HLA-G [mem-G/9], Human IgG1, Fc Silent™, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: mem-G/9
Heavy Chain Modification: Fc Silent™
Synonyms: HLAG; HLA-6.0; HLA-G5; HLAG-6; HLA class I histocompatibility antigen alpha chain G; HLA G antigen; MHC class I antigen G; human leukocyte antigen
Amount: 100 μg
Application Codes Clone: ELISA; FC; IF; IHC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds an epitope in the alpha 1 domain of human HLA-G. It binds the native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. It is also reported to cross react with HLA-G3.
Application Notes: This antibody is not suitable for detecting denatured human HLA-G protein in a western blot. This antibody can be used for the detection of soluble HLA-G in a sample using ELISA (PMID: 10902607). This antibody can effectively detect HLA-G and HLA-G3 on K653-HLA-G1, K562-G3 transductants, M8-HLA-G1 and JEG-3 cells in a flow cytometric analysis (PMID: 12590976; 22738368). This antibody was also used for studying the expression of HLA-G on the surface of JEG-3 cells using immunofluorescence staining (PMID: 22738368). This antibody can be used for immunohistochemical analysis of cryopreserved serial sections of trophoblast tissue. This antibody is not suitable for immunohistochemical staining of paraffin sections (PMID: 12590976). This antibody was also reported to be used in isolation of HLA-G+ cells for prenatal screening (PMID: 35498932).

Product Class:	Purified
Clone ID:	mem-G/9
Heavy Chain Modification:	Fc Silent™
Synonyms:	HLAG; HLA-6.0; HLA-G5; HLAG-6; HLA class I histocompatibility antigen alpha chain G; HLA G antigen; MHC class I antigen G; human leukocyte antigen
Amount:	100 μg
Application Codes Clone:	ELISA; FC; IF; IHC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds an epitope in the alpha 1 domain of human HLA-G. It binds the native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. It is also reported to cross react with HLA-G3.
Application Notes:	This antibody is not suitable for detecting denatured human HLA-G protein in a western blot. This antibody can be used for the detection of soluble HLA-G in a sample using ELISA (PMID: 10902607). This antibody can effectively detect HLA-G and HLA-G3 on K653-HLA-G1, K562-G3 transductants, M8-HLA-G1 and JEG-3 cells in a flow cytometric analysis (PMID: 12590976; 22738368). This antibody was also used for studying the expression of HLA-G on the surface of JEG-3 cells using immunofluorescence staining (PMID: 22738368). This antibody can be used for immunohistochemical analysis of cryopreserved serial sections of trophoblast tissue. This antibody is not suitable for immunohistochemical staining of paraffin sections (PMID: 12590976). This antibody was also reported to be used in isolation of HLA-G+ cells for prenatal screening (PMID: 35498932).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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