Vector Labs Ab03740-1.1 Anti-HLA-E [3D12], Mouse IgG1, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 3D12
Synonyms: HLAE; sHLA-E; HLA-6.2; HLA class I histocompatibility antigen alpha chain E; Major histocompatibility complex class I E; MHC; MHC class I antigen E; MHC HLA E alpha 1; MHC HLA E alpha 2.1; HLAB; HLA-B; HLA-C; HLAC
Amount: 100 μg
Application Codes Clone: FC; functional assay; IF
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody binds an extracellular epitope on the human HLA-E, a member of human leukocyte antigen family. This antibody is capable of reacting with free HLA-E heavy chain as well as heavy chain associated with b2-microglobulin and peptide. This antibody is reported to cross react with HLA-B and HLA-C but not with HLA-A, HLA-F or HLA-G. The human HLA-E is a non-classical MHC class 1B molecule involved in immune self-nonself discrimination. HLA-E plays a very important role in cell recognition by NK cells. Peptide-bound HLA-E-B2M heterotrimeric complex primarily functions as a ligand for natural killer (NK) cell inhibitory receptor KLRD1-KLRC1, enabling NK cells to monitor the expression of other MHC class I molecules in healthy cells and to tolerate self.
Application Notes: The biotin conjugated version of this antibody was used for the study of HLA-E expression and distribution on PBMCs using flow cytometry. This antibody was also used in an assay to study the effect of HLA-E on inhibition of NKL-mediated cytolysis LCL .221 cell line. The presence of 3D11 the resulted in complete restoration of lysis (PMID: 9560253). It was reported that binding of this antibody to HLA-E is inhibited by the peptides sequences 115QFAYDGKDY123 and 137DTAAQI142. It was also reported that this antibody is capable of binding several HLA-Ia antigens like HLA-B and HLA-C (PMID: 21145594). This antibody was used in a study to determined the surface expression of HLA-E in human tumors using immunofluorescent staining and flow cytometry (PMID: 12618909).

Product Class:	Purified
Clone ID:	3D12
Synonyms:	HLAE; sHLA-E; HLA-6.2; HLA class I histocompatibility antigen alpha chain E; Major histocompatibility complex class I E; MHC; MHC class I antigen E; MHC HLA E alpha 1; MHC HLA E alpha 2.1; HLAB; HLA-B; HLA-C; HLAC
Amount:	100 μg
Application Codes Clone:	FC; functional assay; IF
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody binds an extracellular epitope on the human HLA-E, a member of human leukocyte antigen family. This antibody is capable of reacting with free HLA-E heavy chain as well as heavy chain associated with b2-microglobulin and peptide. This antibody is reported to cross react with HLA-B and HLA-C but not with HLA-A, HLA-F or HLA-G. The human HLA-E is a non-classical MHC class 1B molecule involved in immune self-nonself discrimination. HLA-E plays a very important role in cell recognition by NK cells. Peptide-bound HLA-E-B2M heterotrimeric complex primarily functions as a ligand for natural killer (NK) cell inhibitory receptor KLRD1-KLRC1, enabling NK cells to monitor the expression of other MHC class I molecules in healthy cells and to tolerate self.
Application Notes:	The biotin conjugated version of this antibody was used for the study of HLA-E expression and distribution on PBMCs using flow cytometry. This antibody was also used in an assay to study the effect of HLA-E on inhibition of NKL-mediated cytolysis LCL .221 cell line. The presence of 3D11 the resulted in complete restoration of lysis (PMID: 9560253). It was reported that binding of this antibody to HLA-E is inhibited by the peptides sequences 115QFAYDGKDY123 and 137DTAAQI142. It was also reported that this antibody is capable of binding several HLA-Ia antigens like HLA-B and HLA-C (PMID: 21145594). This antibody was used in a study to determined the surface expression of HLA-E in human tumors using immunofluorescent staining and flow cytometry (PMID: 12618909).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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