Mfg Part Number
Ab03742-2.3

Vector Labs Ab03742-2.3 Anti-CD24 [M1/69], Mouse IgG2a, Fc Silent™, kappa (100 μg)

Quick Overview
The original antibody was generated by immunizing DA rats with B10 mouse splenic T cells and concanavalin A-activated splenocytes.
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Product Class:Purified
Clone ID:M1/69
Heavy Chain Modification:Fc Silent™
Synonyms:Heat stable antigen; Signal transducer CD24; Lymphocyte antigen 52; Ly-52; M1/69-J11D heat stable antigen; HSA; Nectadrin; R13-Ag; X62 heat stable antigen
Amount:100 μg
Application Codes Clone:FC; WB; IHC; IF
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG2b
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:This antibody binds mouse CD24 also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a variably glycosylated GPI-anchored membrane protein expressed on the surface lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, and dendritic cells. This protein plays an important role in differentiation of different cell types and its expression varies during lymphocyte differentiation which makes it a good marker for delineating various lymphocyte developmental stages. It is also expressed in erythroleukemia, small cell lung carcinoma and multiple sclerosis.
Application Notes:This antibody was capable of recognizing CD24 antigen from microsomal membrane preparation of different mouse cells through western blot (PMID: 1696896). This antibody is recommended for immunophenotypic analysis of CD24 expressed on mouse B and T cells using flow cytometry (PMID: 17702899; 20133615; 20203098; 33111080). This antibody (dilution 1:100) was also used in the immunohistochemical analysis of CD24 expression in mouse thyroid cells using 5-μm zinc-fixed, paraffin-embedded thyroid sections (PMID: 18801910). This antibody was also reported to be used in the flow cytometric and immunohistochemical analysis of murine CD24 expressed on the surface of normal human breast epithelial cells early after their infection with a retroviral vector (PMID: 9232274). This antibody was also used in the immunofluorescence staining of murine spinal cord sections (PMID: 17475850).
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Brand Vector Labs
UNSPSC 12161500