Mfg Part Number
Ab03805-23.0
Vector Labs Ab03805-23.0 Anti-PD-1 [6#], Rabbit IgG, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing BALB/c mice with PD-1.
$570.70
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | 6# |
| Synonyms: | CD279; Programmed cell death protein 1; PDCD1; PD-1; hPD-1; Human programmed cell death receptor-1 |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; blocking; SPR; inhibition |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | The antibody is specific for PD-1. PD-1 is a type I membrane protein with 288 amino acids, and is one of the main known immune checkpoints. PD-1 is expressed in activated T lymphocytes, and it binds ligands PD-L1 and PD-L2. |
| Application Notes: | The specificity of the original format of the antibody was confirmed by ELISA analysis. The original format and the humanized version of the antibody were capable of blocking the binding of PD-1 and PD-L1. The antibody was able to activate the T cells under in vitro culture conditions and promote the production of IFN-γ, thereby enhancing their T cell immune function. The binding affinity of the murine and humanized versions of the antibody to PD-1 were measured by surface plasmon resonance (Kd= 6.74×10 -10 M and 8.07×10 -10 M respectively). The antibody blocked the interactions between PD-1 and PD-L1. The antibody inhibited tumor growth in NCG mouse models of non-small cell lung cancer and colorectal cancer tumors (CN107043420B). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |