Vector Labs Ab03813-2.0 Anti-Gc [R5], Mouse IgG2a, lambda (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: R5
Synonyms: glycoprotein C; Envelopment polyprotein; M polyprotein
Amount: 100 μg
Application Codes Clone: ELISA; SPR; FACS; Neutralization; in vivo
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for the Gc protein of Rift Valley fever virus (RVFV). The Gc protein is one of the two structural envelope glycoproteins of RVFV found on the virus's surface (the other being the glycoprotein N (Gn) protein). The Gc protein plays a crucial role in the entry of RVFV into host cells.
Application Notes: The original version of this antibody (human IgG1) was characterized using ELISA, SPR, and FACS assays to isolate it and assess its binding specificity and affinity. It bound its target with a K_d of 120 nM. In a FACS-based in vitro neutralization assay, this antibody exhibited a relatively low neutralization potency compared to its Gn-specific antibody counterparts with a median inhibitory concentration (IC₅₀) of 1,370±530 ng/ml. However, it can still prevent RVFV infection at high concentrations. In an in vivo mouse model, mice treated with this antibody showed lower survival rates compared to those treated with Gn-specific antibodies. However, this antibody still contributed to clearing the virus, as evidenced by the survival of one mouse in the respective group, indicating that this antibody can neutralize RVFV infection at high doses also in vivo (Wang et al., 2019; PMID: 30936489).

Product Class:	Purified
Clone ID:	R5
Synonyms:	glycoprotein C; Envelopment polyprotein; M polyprotein
Amount:	100 μg
Application Codes Clone:	ELISA; SPR; FACS; Neutralization; in vivo
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for the Gc protein of Rift Valley fever virus (RVFV). The Gc protein is one of the two structural envelope glycoproteins of RVFV found on the virus's surface (the other being the glycoprotein N (Gn) protein). The Gc protein plays a crucial role in the entry of RVFV into host cells.
Application Notes:	The original version of this antibody (human IgG1) was characterized using ELISA, SPR, and FACS assays to isolate it and assess its binding specificity and affinity. It bound its target with a K_d of 120 nM. In a FACS-based in vitro neutralization assay, this antibody exhibited a relatively low neutralization potency compared to its Gn-specific antibody counterparts with a median inhibitory concentration (IC₅₀) of 1,370±530 ng/ml. However, it can still prevent RVFV infection at high concentrations. In an in vivo mouse model, mice treated with this antibody showed lower survival rates compared to those treated with Gn-specific antibodies. However, this antibody still contributed to clearing the virus, as evidenced by the survival of one mouse in the respective group, indicating that this antibody can neutralize RVFV infection at high doses also in vivo (Wang et al., 2019; PMID: 30936489).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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