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Mfg Part Number

Ab03815-10.3

Vector Labs Ab03815-10.3 Anti-Gn [Mab-1 (23A8-1)], Human IgG1, Fc Silent™, kappa (100 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by immunizing mice with bacterially produced RVFV Gn protein.

$570.70

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Description

Product Class: Purified
Clone ID: Mab-1 (23A8-1)
Heavy Chain Modification: Fc Silent™
Synonyms: RVFV Gn; p78; Glycoprotein G1; Glycoprotein N
Amount: 100 μg
Application Codes Clone: ELISA; Neutralization; in vivo
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: The antibody is specific for the Gn protein binding a linear epitope in the B structural domain (aa 366 to 440). Gn is a surface glycoprotein of RVFV.
Application Notes: The specificity of the original format of the antibody to Gn from RVFV lysate was confirmed by ELISA analysis (EC = 3.9 ng/mL). The antibody was potently neutralizing, with an IC50 of 28 ng/ml. To determine the protective potential of the IgG1 format of the antibody, it was administrated to C57BL/6 mice in a RVFV lethal challenge model. The antibody was able to protect the mice, with only one mouse succumbing to disease. The IgG2a version of the antibody was constructed. It exhibited an RVFV neutralization capacity and ELISA affinity similar to that of its IgG1 counterpart. Further, the IgG2a version showed significantly higher NK cell degranulation. In vivo experiments demonstrated that the IgG2a format of the antibody fully protected mice from the lethal challenge (Cartwright et al., 2021; PMID:34494884).

Product Class:	Purified
Clone ID:	Mab-1 (23A8-1)
Heavy Chain Modification:	Fc Silent™
Synonyms:	RVFV Gn; p78; Glycoprotein G1; Glycoprotein N
Amount:	100 μg
Application Codes Clone:	ELISA; Neutralization; in vivo
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	The antibody is specific for the Gn protein binding a linear epitope in the B structural domain (aa 366 to 440). Gn is a surface glycoprotein of RVFV.
Application Notes:	The specificity of the original format of the antibody to Gn from RVFV lysate was confirmed by ELISA analysis (EC = 3.9 ng/mL). The antibody was potently neutralizing, with an IC50 of 28 ng/ml. To determine the protective potential of the IgG1 format of the antibody, it was administrated to C57BL/6 mice in a RVFV lethal challenge model. The antibody was able to protect the mice, with only one mouse succumbing to disease. The IgG2a version of the antibody was constructed. It exhibited an RVFV neutralization capacity and ELISA affinity similar to that of its IgG1 counterpart. Further, the IgG2a version showed significantly higher NK cell degranulation. In vivo experiments demonstrated that the IgG2a format of the antibody fully protected mice from the lethal challenge (Cartwright et al., 2021; PMID:34494884).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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