Mfg Part Number
Ab03816-1.1
Vector Labs Ab03816-1.1 Anti-Gn [Mab-2 (23B5-1)], Mouse IgG1, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing mice with bacterially produced RVFV Gn protein.
$570.70
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | Mab-2 (23B5-1) |
| Synonyms: | RVFV Gn; p78; Glycoprotein G1; Glycoprotein N |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; Neutralization; in vivo |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | The antibody is specific for the Gn protein binding an epitope in the β1 structural domain (aa 301 to 365). Gn is a surface glycoprotein of RVFV. |
| Application Notes: | The specificity of the original format of the antibody to Gn from RVFV lysate was confirmed by ELISA analysis (EC = 8.8 ng/mL). The antibody was neutralizing, with an IC50 of 1.5 ng/ml. To determine the protective potential of the IgG1 antibody, it was administrated to C57BL/6 mice in a RVFV lethal challenge model. IgG1 isotype control-treated mice succumbed to disease within 3 days of challenge, while the antibody protected mice, with only one mouse succumbing to disease. The IgG2a version of the antibody was constructed. It exhibited an RVFV neutralization capacity and ELISA affinity similar to that of its IgG1 counterpart. Further, the IgG2a version showed significantly higher NK cell degranulation. In vivo experiments demonstrated that the IgG2a format of the antibody fully protected mice from lethal challenge (Cartwright et al., 2021; PMID:34494884). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |