Mfg Part Number
Ab03818-2.0
Vector Labs Ab03818-2.0 Anti-mammalian sTnI [6G1], Mouse IgG2a, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing BALB/c mice with heat-treated TnI in Freund's complete adjuvant, followed by two booster injections with TnI in Freund's incomplete adjuvant.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 6G1 |
| Synonyms: | Skeletal Troponin I; Troponin I; skeletal Troponin C; sTnC |
| Amount: | 100 μg |
| Application Codes Clone: | WB; ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody is specific for mammalian skeletal Troponin I (sTnI) and can cross-react with skeletal Troponin C (sTnC). |
| Application Notes: | This antibody was originally reported to react with equine (horse) Troponin I (TnI) as determined by ELISA (US7297500B2). Later, it was shown through WB to bind mammalian skeletal TNI (sTnI), specifically bovine (cow) and porcine (pig), where it was shown to be immunoreactive to both sTnI and skeletal Troponin C (sTnC). However, sTnC was not detected by this antibody following extraction using urea and βME supplemented extraction buffer, indicating that their addition leads to the destruction of the antibody's epitope on sTnC. In a sandwich ELISA, it was determined that this antibody detects heated bovine, cervine (deer), equine, ovine (sheep), and porcine samples (Jiang et al., 2020; DOI: 10.1016/j.foodcont.2019.107045). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |