Mfg Part Number
Ab03829-23.0
Vector Labs Ab03829-23.0 Anti-MET [7A2], Rabbit IgG, kappa (100 μg)
Quick Overview
The original antibody was selected by biopanning with a scFv phage library performed on solid-phase recombinant MET928.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | 7A2 |
| Synonyms: | human HGF receptor; 084-G02; Hepatocyte growth factor receptor; HGF receptor; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met |
| Amount: | 100 μg |
| Application Codes Clone: | SPR; inhibition; ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | The antibody specifically binds to amino acid residues 568-741 of human MET. |
| Application Notes: | The scFv fragment showed inhibition of HGF/SF induced migration of SKOV cells. In particular, the scFv fragment had an IC50 of 2.3 nM in the SKOV migration assay. The binding affinity of the scFv fragment to MET928 was measured by surface plasmon resonance (Kd= 3.6 nM). In a competition ELISA, binding of 2.5 nM of the Fab version of the antibody to a MET928-coated surface was inhibited by 15 μM and 5 μM NK1 (US20150147274A1). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |