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Mfg Part Number

Ab03836-1.1

Vector Labs Ab03836-1.1 Anti-Severe Fever and Thrombocytopenic Syndrome Virus [MAb4-5], Mouse IgG1, kappa (100 μg)

VECTOR LABS

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Quick Overview

The original antibody was isolated using whole SFTSV virions as bait from a phage-display antibody library derived from the peripheral blood mononuclear cells of a patient that recovered from SFTS disease.

$570.70

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Description

Product Class: Purified
Clone ID: MAb4-5
Synonyms: SFTSV; SFTS; Glycoprotein N; SFTSV Gn; Envelopment polyprotein; Severe Fever and Thrombocytopenic Syndrome Virus; arthropod-borne phleboviruses
Amount: 100 μg
Application Codes Clone: Crystallography; Neutralizing; SPR; WB
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: The antibody is specific for SFTSV. It binds to a linear epitope in the ectodomain of glycoprotein Gn. It does not cross react with RVFV.
Application Notes: The antibody showed neutralizing activity against SFTSV infection in Vero cells (Guo et al. 2013; PMID: 23863504). To verify neutralizing activity, full-length human IgG1 antibody was constructed. The recombinant antibodies were expressed by 293T mammalian cells and the concentration required to obtain 50% neutralization of 100 TCID50 SFTSV in vitro was 44.2 μg/mL. The interaction between Gn and the antibody was further demonstrated by surface plasmon resonance (SPR) assays with a dissociation constant (Kd) of 25.9 nM. The crystal structure of SFTSV Gn head domain in complex with the Fab fragment of the antibody was determined. The antibody could recognize the denatured SFTSV Gn head by WB (Wu et al., 2017; PMID: 28827346).

Product Class:	Purified
Clone ID:	MAb4-5
Synonyms:	SFTSV; SFTS; Glycoprotein N; SFTSV Gn; Envelopment polyprotein; Severe Fever and Thrombocytopenic Syndrome Virus; arthropod-borne phleboviruses
Amount:	100 μg
Application Codes Clone:	Crystallography; Neutralizing; SPR; WB
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	The antibody is specific for SFTSV. It binds to a linear epitope in the ectodomain of glycoprotein Gn. It does not cross react with RVFV.
Application Notes:	The antibody showed neutralizing activity against SFTSV infection in Vero cells (Guo et al. 2013; PMID: 23863504). To verify neutralizing activity, full-length human IgG1 antibody was constructed. The recombinant antibodies were expressed by 293T mammalian cells and the concentration required to obtain 50% neutralization of 100 TCID50 SFTSV in vitro was 44.2 μg/mL. The interaction between Gn and the antibody was further demonstrated by surface plasmon resonance (SPR) assays with a dissociation constant (Kd) of 25.9 nM. The crystal structure of SFTSV Gn head domain in complex with the Fab fragment of the antibody was determined. The antibody could recognize the denatured SFTSV Gn head by WB (Wu et al., 2017; PMID: 28827346).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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