Mfg Part Number
Ab03836-10.3
Vector Labs Ab03836-10.3 Anti-Severe Fever and Thrombocytopenic Syndrome Virus [MAb4-5], Human IgG1, Fc Silent™, kappa (100 μg)
Quick Overview
The original antibody was isolated using whole SFTSV virions as bait from a phage-display antibody library derived from the peripheral blood mononuclear cells of a patient that recovered from SFTS disease.
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| Product Class: | Purified |
|---|---|
| Clone ID: | MAb4-5 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | SFTSV; SFTS; Glycoprotein N; SFTSV Gn; Envelopment polyprotein; Severe Fever and Thrombocytopenic Syndrome Virus; arthropod-borne phleboviruses |
| Amount: | 100 μg |
| Application Codes Clone: | Crystallography; Neutralizing; SPR; WB |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | The antibody is specific for SFTSV. It binds to a linear epitope in the ectodomain of glycoprotein Gn. It does not cross react with RVFV. |
| Application Notes: | The antibody showed neutralizing activity against SFTSV infection in Vero cells (Guo et al. 2013; PMID: 23863504). To verify neutralizing activity, full-length human IgG1 antibody was constructed. The recombinant antibodies were expressed by 293T mammalian cells and the concentration required to obtain 50% neutralization of 100 TCID50 SFTSV in vitro was 44.2 μg/mL. The interaction between Gn and the antibody was further demonstrated by surface plasmon resonance (SPR) assays with a dissociation constant (Kd) of 25.9 nM. The crystal structure of SFTSV Gn head domain in complex with the Fab fragment of the antibody was determined. The antibody could recognize the denatured SFTSV Gn head by WB (Wu et al., 2017; PMID: 28827346). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |