Vector Labs Ab03839-10.3 Anti-Hepatocyte growth factor [t1E4], Human IgG1, Fc Silent™, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: t1E4
Heavy Chain Modification: Fc Silent™
Synonyms: HGF; HPTA; Hepatopoietin-A; Scatter factor
Amount: 100 μg
Application Codes Clone: ELISA; inhibition; IP; SPR
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2b
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: The antibody is specific for HGF. It can bind to the two chains and to the single chain HGF. It recognizes an epitope located within the K4-SP region.
Application Notes: The specificity of the original format of the antibody for HGF was confirmed by ELISA analysis. The authors investigated if the antibody has any effect on the HGF-Met signaling. Met activation was strongly inhibited by the antibody, with an IC50 value of ~0.15 μg/ml or 0.1nM. In particular, antibody caused dose dependent reduction of the HGF-induced phosphorylation levels of Met (Tyr1234/1235), Akt (Ser473), and ERK (Thr202 and Tyr204). The inhibitory action of the antibody was further evaluated by two cell-based assays. The effect of all antibody on the HGF-induced scattering of Madin-Darby canine kidney (MDCK) cells was evaluated, and confirmed that only it could strongly suppress the scattering behavior of the cells when added at 10 μg/ml. Furthermore, the antibody was able to inhibit the HGF-stimulated migration of human liver bile duct carcinoma cells with a similar concentration dependency as the inhibition of Met phosphorylation in EHMES-1 cells. The antibody immunoprecipitated both scHGF and tcHGF. The binding affinity of the antibody toward the HGF proteins was tested by SPR.

Product Class:	Purified
Clone ID:	t1E4
Heavy Chain Modification:	Fc Silent™
Synonyms:	HGF; HPTA; Hepatopoietin-A; Scatter factor
Amount:	100 μg
Application Codes Clone:	ELISA; inhibition; IP; SPR
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2b
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	The antibody is specific for HGF. It can bind to the two chains and to the single chain HGF. It recognizes an epitope located within the K4-SP region.
Application Notes:	The specificity of the original format of the antibody for HGF was confirmed by ELISA analysis. The authors investigated if the antibody has any effect on the HGF-Met signaling. Met activation was strongly inhibited by the antibody, with an IC50 value of ~0.15 μg/ml or 0.1nM. In particular, antibody caused dose dependent reduction of the HGF-induced phosphorylation levels of Met (Tyr1234/1235), Akt (Ser473), and ERK (Thr202 and Tyr204). The inhibitory action of the antibody was further evaluated by two cell-based assays. The effect of all antibody on the HGF-induced scattering of Madin-Darby canine kidney (MDCK) cells was evaluated, and confirmed that only it could strongly suppress the scattering behavior of the cells when added at 10 μg/ml. Furthermore, the antibody was able to inhibit the HGF-stimulated migration of human liver bile duct carcinoma cells with a similar concentration dependency as the inhibition of Met phosphorylation in EHMES-1 cells. The antibody immunoprecipitated both scHGF and tcHGF. The binding affinity of the antibody toward the HGF proteins was tested by SPR.

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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