Vector Labs Ab03842-1.1 Anti-Hendra and Nipah virus F [1F5], Mouse IgG1, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 1F5
Synonyms: NiV; HeV; NiV F; HeV F; Protein F; F protein; Fusion glycoprotein; Fusion glycoprotein F0; Hendra henipavirus; Nipah henipavirus
Amount: 100 μg
Application Codes Clone: Functional assay; Neutralize
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: Fab
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This reformatted mouse antibody was made using the variable domain sequences of the original Mouse Fab format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds a pre-fusion conformational epitope on the F glycoprotein of the Nipah and Hendra virus. The Nipah and Hendra virus are spread by bats.
Application Notes: This antibody is reported to broadly neutralize Nipah and Hendra virus by blocking membrane fusion. The cryogenic-electron microscopy (cryo-EM) structures of the antibody in complex with HeV F was determined. Biolayer interferometry was used to characterize the binding of the 1F5 Fab fragments to prefusion NiV F or HeV F ectodomain trimers immobilized on the surface of biosensors. The equilibrium dissociation constants (Kd) of 1.47 and 1.24 nM for attachment of Fab fragments of 1F5 to NiV F or HeV F, respectively. To further characterize this antibody, a kinetic assay was performed on HeV F- or NiV F- or NiV F S69A using the mouse version of this antibody. To analyze the ability of this antibody to prevent and cure disease, a fusion inhibition assay was performed on CHO745 cells transfected with HNV F and G glycoproteins using the mouse IgG version of this antibody. Further a plaque reduction assay was performed NiV-M, NiV-B or HeV using both the human and mouse IgG versions of this antibody. The humanized version of the antibody broadly inhibited NiV-M, NiV-B and HeV with IC50 ranging between 0.2 and 1.3 µg/ml (Dang et al., 2021; PMID:33927387).

Product Class:	Purified
Clone ID:	1F5
Synonyms:	NiV; HeV; NiV F; HeV F; Protein F; F protein; Fusion glycoprotein; Fusion glycoprotein F0; Hendra henipavirus; Nipah henipavirus
Amount:	100 μg
Application Codes Clone:	Functional assay; Neutralize
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	Fab
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This reformatted mouse antibody was made using the variable domain sequences of the original Mouse Fab format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds a pre-fusion conformational epitope on the F glycoprotein of the Nipah and Hendra virus. The Nipah and Hendra virus are spread by bats.
Application Notes:	This antibody is reported to broadly neutralize Nipah and Hendra virus by blocking membrane fusion. The cryogenic-electron microscopy (cryo-EM) structures of the antibody in complex with HeV F was determined. Biolayer interferometry was used to characterize the binding of the 1F5 Fab fragments to prefusion NiV F or HeV F ectodomain trimers immobilized on the surface of biosensors. The equilibrium dissociation constants (Kd) of 1.47 and 1.24 nM for attachment of Fab fragments of 1F5 to NiV F or HeV F, respectively. To further characterize this antibody, a kinetic assay was performed on HeV F- or NiV F- or NiV F S69A using the mouse version of this antibody. To analyze the ability of this antibody to prevent and cure disease, a fusion inhibition assay was performed on CHO745 cells transfected with HNV F and G glycoproteins using the mouse IgG version of this antibody. Further a plaque reduction assay was performed NiV-M, NiV-B or HeV using both the human and mouse IgG versions of this antibody. The humanized version of the antibody broadly inhibited NiV-M, NiV-B and HeV with IC50 ranging between 0.2 and 1.3 µg/ml (Dang et al., 2021; PMID:33927387).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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