Mfg Part Number
Ab03850-7.4
Vector Labs Ab03850-7.4 Anti-MAdCAM-1 [MECA-89], Rat IgG2a, Fc Silent™, kappa (100 μg)
Quick Overview
Endothelial cells isolated from BALB/c mouse mesenteric and peripheral lymph nodes.
$570.70
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | MECA-89 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | ATCC HB-292; Addressin; mucosal vascular addressin cell adhesion molecule 1 |
| Amount: | 100 μg |
| Application Codes Clone: | IHC; Block |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | The antibody is specific for mouse MAdCAM-1. The antibody recognizes the second immunoglobulin domain, interfering with L-selectin interactions of MAdCAM-1. The mucosal lymphocyte homing receptor MAdCAM-1 is expressed in the high endothelial venules of the mesenteric lymph nodes (MLN) and Peyer's patches (PP) and in the flat epithelium of the lamina propria (LP). |
| Application Notes: | The antibody bound to the second domain of MAdCAM-1 and did not block MAdCAM-1-dependent binding in vitro (Streeter et al., 1988; PMID:3340147). The antibody was used for immunohistochemical analysis of healthy AKR control mice and it showed faint basal expression of MAdCAM-1 in the terminal ileum (Bachmann et al. 2006). The antibody blocked interactions with L-selectin in vivo (Bargatze et al., 1995; PMID: 7542550). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |