Mfg Part Number
Ab03855-23.0

Vector Labs Ab03855-23.0 Anti-HIV p24 antibody; HIV gp120 antibody [1F7], Rabbit IgG, kappa (100 μg)

Quick Overview
The original antibody was generated by immunizing BALB/c mice with pooled human polyclonal HIVIG.
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Product Class:Purified
Clone ID:1F7
Synonyms:ATCC No. HB 11286; anti-idiotypic antibody
Amount:100 μg
Application Codes Clone:WB; ELISA; in vivo
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgM
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:The anti-idiotypic antibody recognizes antibodies that react to different components of the HIV virus, such as gp120, p24. The antibody does not react with antibodies derived from SLE and B cell lymphoma.
Application Notes:The HIV-1 antigen capture ELISA was used to test the binding of human monoclonal anti-HIV-1 to the antibody. The antibody bound to anti-p24 antibodies and anti-gp120 antibodies. Western blot analysis confirmed that the antibody reacts with human antibodies of different HIV-1 antigen specificities (Wang et al., 1992; PMID: 1378015). SHIV-infected macaques were immunized with the antibody. Significant increases in breadth and potency of HIV-1 neutralizing antibody titers to one or more virus strains were detected in all three of the 1F7 immunized macaques (US6057421A).
More Information
Brand Vector Labs
UNSPSC 12161500