Mfg Part Number
Ab03857-10.0

Vector Labs Ab03857-10.0 Anti-CD28 [PV1], Human IgG1, kappa (100 μg)

Quick Overview
The antibody was generated using C57BL/6N mouse T cell lymphoma EL4 cell line as antigen.
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Product Class:Purified
Clone ID:PV1
Synonyms:T-cell-specific surface glycoprotein CD28; CD28 receptor
Amount:100 μg
Application Codes Clone:FC; IP
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:The antibody binds specifically to murine CD28.
Application Notes:CD28-positive murine T cell line EL4 cells were stained with the antibody and analyzed by flow cytometry (US20040116675A1). Protein lysates from J558 were immunoprecipitated with the antibody (Rozanski et al., 2015; PMID: 25833397). Anti-CD28-PV1-IgG3 is the hamster-mouse chimeric version of the antibody. The antibody enabled long-term survival of heart allografts in rats by reducing the activation of alloantigen-mediated key signaling events in T cells (Jang et al. 2008; PMID: 18408588).
More Information
Brand Vector Labs
UNSPSC 12161500