Vector Labs Ab03871-10.3 Anti-IDH1 mutant [MsMab-1], Human IgG1, Fc Silent™, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: MsMab-1
Heavy Chain Modification: Fc Silent™
Synonyms: IDH; IDH1; IDPc; Isocitrate dehydrogenase [NADP] cytoplasmic; Isocitrate dehydrogenase 1; Cytosolic NADP-isocitrate dehydrogenase; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase; IDH2; Isocitrate dehydrogenase [NADP]; mitochondrial; ICD-M; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase
Amount: 100 μg
Application Codes Clone: ELISA; WB; IHC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds human isocitrate dehydrogenase 1 (IDH1) having R132H, R132C, R132S, R132G and R132L mutations and does not cross react with wild-type IDH1 protein. It also recognizes IDH2-R172M (GGTKPITIGMHAHGDQYKA) and IDH2-R172S (GGTKPITIGSHAHGDQYKA). IDH1 is an enzyme that catalyzes the NADP+-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase. It plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways. The NADPH produced from isocitrate dehydrogenase 1 is involved in the breakdown of fats for energy, and it also protects cells from potentially harmful molecules called reactive oxygen species. Mutation in IDH1 are found in several genetic conditions and in some types of cancer, including gliomas, cartilaginous tumors, acute myelogenous leukemia, myelodysplastic syndromes, and brain cancer.
Application Notes: The binding reactivity of this antibody to wild-type IDH1 and its mutants (R132H, R132C, R132S, R132G and R132L) peptides was determined using ELISA. Western blot analysis also revealed that MsMab-1 reacted with recombinant proteins of IDH1-R132H, IDH1-R132S, and IDH1-R132G, but not with wild type IDH1 and other IDH1 mutations. This antibody also recognizes IDH2-R172M protein, despite that the IDH1-R132G peptide shows only 73.7% identity with the equivalent portion of IDH2-R172M (GGTKPITIGMHAHGDQYKA). This antibody successfully stained the IDH1-R132S or IDH1-R132G-expressing glioma cells in immunohistochemistry (PMID: 23782684). This antibody can bind IDH2-R172S mutant of IDH2 in an ELISA and can recognize PA-tagged IDH2-R172S recombinant protein in a western blot. This antibody was also used to stain IDH2-R172S-expressing osteosarcoma tissues in immunohistochemistry (PMID: 24403254). A complete summary of the reactivity of this antibody towards various IDH1 and IDH2 mutants can be found in Liu et. al, 2013 (PMID: 24403254).

Product Class:	Purified
Clone ID:	MsMab-1
Heavy Chain Modification:	Fc Silent™
Synonyms:	IDH; IDH1; IDPc; Isocitrate dehydrogenase [NADP] cytoplasmic; Isocitrate dehydrogenase 1; Cytosolic NADP-isocitrate dehydrogenase; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase; IDH2; Isocitrate dehydrogenase [NADP]; mitochondrial; ICD-M; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase
Amount:	100 μg
Application Codes Clone:	ELISA; WB; IHC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds human isocitrate dehydrogenase 1 (IDH1) having R132H, R132C, R132S, R132G and R132L mutations and does not cross react with wild-type IDH1 protein. It also recognizes IDH2-R172M (GGTKPITIGMHAHGDQYKA) and IDH2-R172S (GGTKPITIGSHAHGDQYKA). IDH1 is an enzyme that catalyzes the NADP+-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase. It plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways. The NADPH produced from isocitrate dehydrogenase 1 is involved in the breakdown of fats for energy, and it also protects cells from potentially harmful molecules called reactive oxygen species. Mutation in IDH1 are found in several genetic conditions and in some types of cancer, including gliomas, cartilaginous tumors, acute myelogenous leukemia, myelodysplastic syndromes, and brain cancer.
Application Notes:	The binding reactivity of this antibody to wild-type IDH1 and its mutants (R132H, R132C, R132S, R132G and R132L) peptides was determined using ELISA. Western blot analysis also revealed that MsMab-1 reacted with recombinant proteins of IDH1-R132H, IDH1-R132S, and IDH1-R132G, but not with wild type IDH1 and other IDH1 mutations. This antibody also recognizes IDH2-R172M protein, despite that the IDH1-R132G peptide shows only 73.7% identity with the equivalent portion of IDH2-R172M (GGTKPITIGMHAHGDQYKA). This antibody successfully stained the IDH1-R132S or IDH1-R132G-expressing glioma cells in immunohistochemistry (PMID: 23782684). This antibody can bind IDH2-R172S mutant of IDH2 in an ELISA and can recognize PA-tagged IDH2-R172S recombinant protein in a western blot. This antibody was also used to stain IDH2-R172S-expressing osteosarcoma tissues in immunohistochemistry (PMID: 24403254). A complete summary of the reactivity of this antibody towards various IDH1 and IDH2 mutants can be found in Liu et. al, 2013 (PMID: 24403254).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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