Mfg Part Number
Ab03874-23.0

Vector Labs Ab03874-23.0 Anti-CD277 [108.5], Rabbit IgG, kappa (100 μg)

Quick Overview
The original antibody was generated by immunizing BALB/c mice with soluble BT3.1-Ig fusion protein.
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Product Class:Purified
Clone ID:108.5
Synonyms:BTN3A1; BTF5; BT3; BT3.1; Butyrophilin subfamily 3 member A1
Amount:100 μg
Application Codes Clone:antagonist; functional assay
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG1
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:This antibody binds human CD277 (BTNA3A1). This protein plays an important role in T-cell activation and in the adaptive immune response. It regulates proliferation of activated T-cells and release of IFNG by activated T-cells. It is expressed on surface of T-cells, natural killer cells, dendritic cells and macrophages (at protein level). It is highly expressed in heart, pancreas and lung and moderately expressed in placenta, liver and muscle.
Application Notes:This antibody was used as an antagonist antibody to check if BTN3A can specifically enhance Vg9Vd2 TCR-mediated lysis of primary AML blasts using cytotoxic assays. This antibody was reported to significantly and drastically abrogate zoledronate (ZOL)-induced lysis (PMID: 27853633). This antibody can completely abrogate Vγ9Vδ2 T cell mediated lysis of tumors (PMID: 29296524).
More Information
Brand Vector Labs
UNSPSC 12161500