Vector Labs Ab03875-10.3 Anti-p21Ras [KGH-R1], Human IgG1, Fc Silent™, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: KGH-R1
Heavy Chain Modification: Fc Silent™
Synonyms: p21; p21 ras protein; Ras; pan ras; K-Ras; GTPase Kras; KRAS2; RASK2; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras; ras p21 protein; p21 ras; GTPase Hras; HRAS; H-RAS; HRAS; HRAS1; GTPase HRas; H-Ras-1; Ha-Ras; Transforming protein p21; c-H-ras; p21ras; N-RAS; NRAS; HRAS1; GTPase NRas; Transforming protein N-Ras
Amount: 100 μg
Application Codes Clone: ELISA; WB; ICC; IHC-FFPE; IF
Shipping Temperature: Wet Ice
Origin Pub PMID: 26897358
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2b
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody recognizes human wild-type and mutant H-, K-, and N-Ras proteins. This ras protein binds GDP/GTP and possess intrinsic GTPase activity, that results in conversion of nucleotide guanosine triphosphate (GTP) into guanosine diphosphate (GDP). This protein plays an important role in many signal transduction pathways and regulation of cell proliferation. Somatic ras mutations are highly observed in leukemias, colorectal cancer, pancreatic cancer and lung cancer.
Application Notes: The binding reactivity of this antibody to H-p21Ras, K-p21Ras and N-p21Ras protein was determined using ELISA. This antibody was also capable of recognizing wild type and mutated ras protein from various normal and tumor cell lines using western blot and immunocytometry assay. This antibody presented strong immunoreactivity for 14 primary human cancer tissues but showed weak or negative immunoreactivity in the corresponding normal tissues. It was reported to bind positively with QGY7703, SMMC7721, HepG2: hepatocarcinoma; BGC853, MKN28: gastric cancer; HCT116: colorectal cancer; T24: bladder cancer; SKOV3: ovary cancer; MDA-MB-231, MDA-MB-435, MCF7: breast cancer; HeLa: cervical cancer; Hep2: laryngocarcinoma; C8166, K562 cell lines (PMID: 26897358). This antibody was also used in the immunohistochemical staining of FFPE section of colorectal tissues, colorectal inflammatory polyps, colorectal low-grade intraepithelial neoplasia, colorectal high-grade intraepithelial neoplasia, invasive colorectal carcinomas and corresponding adjacent tissues. The immunoreactivity was measured by evaluating percentage of positive cells and histological score (HSCORE) (PMID: 21138685). This antibody can also be used in the immunofluorescent staining of various normal and cancer tissues (PMID: 32664770).

Product Class:	Purified
Clone ID:	KGH-R1
Heavy Chain Modification:	Fc Silent™
Synonyms:	p21; p21 ras protein; Ras; pan ras; K-Ras; GTPase Kras; KRAS2; RASK2; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras; ras p21 protein; p21 ras; GTPase Hras; HRAS; H-RAS; HRAS; HRAS1; GTPase HRas; H-Ras-1; Ha-Ras; Transforming protein p21; c-H-ras; p21ras; N-RAS; NRAS; HRAS1; GTPase NRas; Transforming protein N-Ras
Amount:	100 μg
Application Codes Clone:	ELISA; WB; ICC; IHC-FFPE; IF
Shipping Temperature:	Wet Ice
Origin Pub PMID:	26897358
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2b
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody recognizes human wild-type and mutant H-, K-, and N-Ras proteins. This ras protein binds GDP/GTP and possess intrinsic GTPase activity, that results in conversion of nucleotide guanosine triphosphate (GTP) into guanosine diphosphate (GDP). This protein plays an important role in many signal transduction pathways and regulation of cell proliferation. Somatic ras mutations are highly observed in leukemias, colorectal cancer, pancreatic cancer and lung cancer.
Application Notes:	The binding reactivity of this antibody to H-p21Ras, K-p21Ras and N-p21Ras protein was determined using ELISA. This antibody was also capable of recognizing wild type and mutated ras protein from various normal and tumor cell lines using western blot and immunocytometry assay. This antibody presented strong immunoreactivity for 14 primary human cancer tissues but showed weak or negative immunoreactivity in the corresponding normal tissues. It was reported to bind positively with QGY7703, SMMC7721, HepG2: hepatocarcinoma; BGC853, MKN28: gastric cancer; HCT116: colorectal cancer; T24: bladder cancer; SKOV3: ovary cancer; MDA-MB-231, MDA-MB-435, MCF7: breast cancer; HeLa: cervical cancer; Hep2: laryngocarcinoma; C8166, K562 cell lines (PMID: 26897358). This antibody was also used in the immunohistochemical staining of FFPE section of colorectal tissues, colorectal inflammatory polyps, colorectal low-grade intraepithelial neoplasia, colorectal high-grade intraepithelial neoplasia, invasive colorectal carcinomas and corresponding adjacent tissues. The immunoreactivity was measured by evaluating percentage of positive cells and histological score (HSCORE) (PMID: 21138685). This antibody can also be used in the immunofluorescent staining of various normal and cancer tissues (PMID: 32664770).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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