Vector Labs Ab03883-2.3 Anti-DPGA anthrax [11D ], Mouse IgG2a, Fc Silent™, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 11D
Heavy Chain Modification: Fc Silent™
Synonyms: DPGA; capsular antigen; γDPGA; gamma DPGA; poly-gamma-d-glutamic acid; poly-γ-d-glutamic acid; antiphagocytic polypeptide capsule
Amount: 100 μg
Application Codes Clone: ELISA; neutralize; in vivo
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Chimpanzee IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody specifically binds the poly-γ-D-glutamic acid (γDPGA) capsule of Bacillus anthracis. Bacillus anthracis is surrounded by an antiphagocytic polypeptide capsule composed of poly γ-d-glutamic acid (γDPGA). The γDPGA capsule shields the vegetative form of B. anthracis from agglutination by monoclonal antibodies to its cell wall polysaccharide. γDPGA has been identified recently as a potential target for vaccine development. Bacillus anthracis is a spore-forming bacterium and a causative agent for Anthrax, which is a highly lethal infectious disease in human and poses a great threat as an emerging bioterror agent.
Application Notes: The binding characterization of this antibody to γDPGA of Bacillus anthracis was done using ELISA. The binding affinity of the antibody was measured using surface plasmon resonance. It was reported that the IgG1 version of this antibody binds 10-mer peptide of γDPGA with a binding affinity of Kd= 0.3 nM. It was reported that a single 30-μg dose of this antibody when given to BALB/c mice 18 h before challenge conferred about 50% protection against a lethal intratracheal spore challenge by the virulent B. anthracis Ames strain. When given 8 h or 20 h after challenge, this antibody provided significant protection against lethal infection (PMID: 21187383). This antibody can be used in treatment of infections with antibiotic-resistant strains (PMID: 22069754).

Product Class:	Purified
Clone ID:	11D
Heavy Chain Modification:	Fc Silent™
Synonyms:	DPGA; capsular antigen; γDPGA; gamma DPGA; poly-gamma-d-glutamic acid; poly-γ-d-glutamic acid; antiphagocytic polypeptide capsule
Amount:	100 μg
Application Codes Clone:	ELISA; neutralize; in vivo
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric mouse antibody was made using the variable domain sequences of the original Chimpanzee IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody specifically binds the poly-γ-D-glutamic acid (γDPGA) capsule of Bacillus anthracis. Bacillus anthracis is surrounded by an antiphagocytic polypeptide capsule composed of poly γ-d-glutamic acid (γDPGA). The γDPGA capsule shields the vegetative form of B. anthracis from agglutination by monoclonal antibodies to its cell wall polysaccharide. γDPGA has been identified recently as a potential target for vaccine development. Bacillus anthracis is a spore-forming bacterium and a causative agent for Anthrax, which is a highly lethal infectious disease in human and poses a great threat as an emerging bioterror agent.
Application Notes:	The binding characterization of this antibody to γDPGA of Bacillus anthracis was done using ELISA. The binding affinity of the antibody was measured using surface plasmon resonance. It was reported that the IgG1 version of this antibody binds 10-mer peptide of γDPGA with a binding affinity of Kd= 0.3 nM. It was reported that a single 30-μg dose of this antibody when given to BALB/c mice 18 h before challenge conferred about 50% protection against a lethal intratracheal spore challenge by the virulent B. anthracis Ames strain. When given 8 h or 20 h after challenge, this antibody provided significant protection against lethal infection (PMID: 21187383). This antibody can be used in treatment of infections with antibiotic-resistant strains (PMID: 22069754).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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