Vector Labs Ab03885-10.3 Anti-DPGA anthrax [F24F2], Human IgG1, Fc Silent™, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: F24F2
Heavy Chain Modification: Fc Silent™
Synonyms: DPGA; capsular antigen; γDPGA; gamma DPGA; poly-gamma-d-glutamic acid; poly-γ-d-glutamic acid; antiphagocytic polypeptide capsule
Amount: 100 μg
Application Codes Clone: ELISA; IHC; ICC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG3
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG3 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody specifically binds the poly-γ-D-glutamic acid (γDPGA) capsule of Bacillus anthracis. Bacillus anthracis is surrounded by an antiphagocytic polypeptide capsule composed of poly γ-d-glutamic acid (γDPGA). The γDPGA capsule shields the vegetative form of B. anthracis from agglutination by monoclonal antibodies to its cell wall polysaccharide. γDPGA has been identified recently as a potential target for vaccine development. Bacillus anthracis is a spore-forming bacterium and a causative agent for Anthrax, which is a highly lethal infectious disease in human and poses a great threat as an emerging bioterror agent.
Application Notes: This antibody was used for development of a quantitative capture ELISA for PDGA (PMID: 18195035). A 50:50 mixture of PDGA antibodies F24F2 and F26G3 was used in the development of an antigen capture ELISA with a sensitivity of approximately 9 ng/ml of serum after a 1/40 dilution and 2.25 ng/ml for a 1:10 dilution (PMID: 19506008). The HRP or Alexa Fluor 555 labelled antibody was also used in the immunohistochemical staining of γDPGA in mouse liver and spleen tissues after they were injected with 500, 100, or 20µg of γDPGA and tissues were harvested on days 1, 2, 4, and 8 (PMID: 18195035). This antibody was also used in the cellular localization of γDPGA in mouse liver (PMID: 18195035).

Product Class:	Purified
Clone ID:	F24F2
Heavy Chain Modification:	Fc Silent™
Synonyms:	DPGA; capsular antigen; γDPGA; gamma DPGA; poly-gamma-d-glutamic acid; poly-γ-d-glutamic acid; antiphagocytic polypeptide capsule
Amount:	100 μg
Application Codes Clone:	ELISA; IHC; ICC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG3
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG3 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody specifically binds the poly-γ-D-glutamic acid (γDPGA) capsule of Bacillus anthracis. Bacillus anthracis is surrounded by an antiphagocytic polypeptide capsule composed of poly γ-d-glutamic acid (γDPGA). The γDPGA capsule shields the vegetative form of B. anthracis from agglutination by monoclonal antibodies to its cell wall polysaccharide. γDPGA has been identified recently as a potential target for vaccine development. Bacillus anthracis is a spore-forming bacterium and a causative agent for Anthrax, which is a highly lethal infectious disease in human and poses a great threat as an emerging bioterror agent.
Application Notes:	This antibody was used for development of a quantitative capture ELISA for PDGA (PMID: 18195035). A 50:50 mixture of PDGA antibodies F24F2 and F26G3 was used in the development of an antigen capture ELISA with a sensitivity of approximately 9 ng/ml of serum after a 1/40 dilution and 2.25 ng/ml for a 1:10 dilution (PMID: 19506008). The HRP or Alexa Fluor 555 labelled antibody was also used in the immunohistochemical staining of γDPGA in mouse liver and spleen tissues after they were injected with 500, 100, or 20µg of γDPGA and tissues were harvested on days 1, 2, 4, and 8 (PMID: 18195035). This antibody was also used in the cellular localization of γDPGA in mouse liver (PMID: 18195035).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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