Mfg Part Number
Ab03893-1.1
Vector Labs Ab03893-1.1 Anti-Factor H binding protein [clone4], Mouse IgG1, kappa (100 μg)
Quick Overview
The recombinant fHbp protein of Neisseria meningitidis was used as the antigen for the immunization of BALB/c mice. Anti-fHbp VH/k chain ribosome display libraries was generated and the actual antibody was obtained by panning the scFv library against the recombinant fHbp protein by using a single round of the ribosome display method via a rabbit reticulocyte lysate system.
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| Product Class: | Purified |
|---|---|
| Clone ID: | clone4 |
| Synonyms: | fHbp; GNA1870; Genome-derived Neisseria antigen 1870; Lipoprotein 2086 |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length, reformatted mouse antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody binds a surface exposed epitope on N. meningitidis FHbp, a virulence factor critical for surviving of the bacteria in the human host. FHbp protein is a bacterial surface lipoprotein that binds host (human) complement factor H (fH, gene CFH). Neisseria meningitidis is the leading global cause of meningitis and sepsis. |
| Application Notes: | This antibody exhibited high affinity and specificity in the enzyme-linked immunosorbent assay (ELISA) and the whole bacterial cell enzyme-linked immunosorbent assay (Bact-ELISA). The binding affinity of the scFv version of this antibody for fHbp was reported to be Kd= 7.03 nM (PMID: 29737965). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |