Vector Labs Ab03894-23.0 Anti-Factor H binding protein [JAR41], Rabbit IgG, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: JAR41
Synonyms: fHbp; GNA1870; Genome-derived Neisseria antigen 1870; Lipoprotein 2086; JAR 41
Amount: 100 μg
Application Codes Clone: ELISA; FC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds an epitope located on a conserved region of N-terminal domain of N. meningitidis FHbp, a virulence factor critical for surviving of the bacteria in the human host. This antibody binds equally well to fHbp variants, including representative variants in variant groups 1, 2 or 3. FHbp protein is a bacterial surface lipoprotein that binds host (human) complement factor H (fH, gene CFH). Neisseria meningitidis is the leading global cause of meningitis and sepsis.
Application Notes: This antibody broadly cross-reacts with fHbp sequence variants from all N. meningitidis variant groups. In combination with second non-bactericidal anti-fHbp mAb like JAR 5, JAR 41 elicited complement-mediated bactericidal activity in vitro, and augmented passive protection against meningococcal bacteremia in human fH transgenic rats (PMID: 22461972). JAR 41 shows concentration-dependent binding to fHbp ID 1 (variant group 1), fHbp ID 77 (variant group 2) and fHbp ID 28 (variant group 3) in an ELISA. This antibody specifically binds fHbp and does not cross react with other meningococcal surface proteins like NadA and GNA1030. The ability of this antibody to bind 21 fHbp sequence variants was also measured by ELISA. Flow cytometry was used to identify binding of this antibody to surface of live bacteria from N. meningitidis strains (PMID: 22461972). It was also found that JAR 41 augments passive protective activity of anti-fHbp mAb JAR 5 against bacteremia caused by group B strain H44/76 in human fH transgenic infant rats (PMID: 22461972).

Product Class:	Purified
Clone ID:	JAR41
Synonyms:	fHbp; GNA1870; Genome-derived Neisseria antigen 1870; Lipoprotein 2086; JAR 41
Amount:	100 μg
Application Codes Clone:	ELISA; FC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds an epitope located on a conserved region of N-terminal domain of N. meningitidis FHbp, a virulence factor critical for surviving of the bacteria in the human host. This antibody binds equally well to fHbp variants, including representative variants in variant groups 1, 2 or 3. FHbp protein is a bacterial surface lipoprotein that binds host (human) complement factor H (fH, gene CFH). Neisseria meningitidis is the leading global cause of meningitis and sepsis.
Application Notes:	This antibody broadly cross-reacts with fHbp sequence variants from all N. meningitidis variant groups. In combination with second non-bactericidal anti-fHbp mAb like JAR 5, JAR 41 elicited complement-mediated bactericidal activity in vitro, and augmented passive protection against meningococcal bacteremia in human fH transgenic rats (PMID: 22461972). JAR 41 shows concentration-dependent binding to fHbp ID 1 (variant group 1), fHbp ID 77 (variant group 2) and fHbp ID 28 (variant group 3) in an ELISA. This antibody specifically binds fHbp and does not cross react with other meningococcal surface proteins like NadA and GNA1030. The ability of this antibody to bind 21 fHbp sequence variants was also measured by ELISA. Flow cytometry was used to identify binding of this antibody to surface of live bacteria from N. meningitidis strains (PMID: 22461972). It was also found that JAR 41 augments passive protective activity of anti-fHbp mAb JAR 5 against bacteremia caused by group B strain H44/76 in human fH transgenic infant rats (PMID: 22461972).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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