Mfg Part Number
Ab03911-6.1
Vector Labs Ab03911-6.1 Anti-Vimentin [YT33], Rat IgG1, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing BDF1 mice with keyhole limpet hemocyanin (KLH) coupled PV33 — a synthesized vimentin peptide containing the ser33 phosphorylation — (PV33-KLH) emulsified in complete Freund's adjuvant.
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| Product Class: | Purified |
|---|---|
| Clone ID: | YT33 |
| Amount: | 100 μg |
| Application Codes Clone: | IF; WB; ICC; IEM; IHC-p; ELISA |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 8605039 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody specifically recognizes vimentin phosphorylation at Ser33, a C kinase phosphorylation site. |
| Application Notes: | This antibody is specific to the C kinase phosphorylated vimentin Ser33 residue, as determined by ELISA, can detect the activity of endogenous CAM kinase II astrocytes, as detected by WB, and was used for ICC (IF) to detect C kinase phosphorylated vimentin Ser33 in type 1 astrocytes extracted from newborn rats' cerebral cortices (Ogawara et al., 1995; PMID: 7490282). This antibody was used for: in vivo vimentin kinase activity visualization of vimentin kinase activity in cell signaling or mitosis (Goto et al., 1998; PMID: 9565595), IHC-p and IF staining of human glioma cells (Fujita et al., 2004; PMID: 15597762), and as a positive control in a WB for vimentin ser55 phosphorylation (Yamaguchi et al., 2005; PMID: 16260496). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |