Mfg Part Number
Ab03913-2.3
Vector Labs Ab03913-2.3 Anti-VSV-G [M55], Mouse IgG2a, Fc Silent™, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing SCID BALB/c mice with vesicular stomatitis virus (VSV).
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| Product Class: | Purified |
|---|---|
| Clone ID: | M55 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | Glycoprotein G; Glycoprotein; G |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; Neutralization |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody reacts with a defined subsite within the major antigenic site of VSV-G. The exact epitope is not defined. |
| Application Notes: | This stable, recombinant single-chain Fv-Cκ (scFv-Cκ) antibody fragment was generated by protoplast fusion of J558L myeloma cells with Escherichia coli K803 transformed with an appropriate scFv-Cκ expression construct. The aptly named M55 scFv-Cκ expression construct — containing a CDR2 Ser55-to-Arg substitution (T-to-G transition at the third base), found in the VH chain of several hypermutated antibodies (VI22, VI43, and VI53) — was derived from the secondary antibody clone VI24. M55 binds a defined subsite within the major antigenic site of VSV-G. However, the exact binding site in the subunit is not defined. M55 binding to VSV-G was measured using solid-phase ELISA. The VH Ser55-to-Arg substitution in M55, which is not found in its parental clone VI24, increased monovalent binding by about 10-fold compared with M0 (the germline derived scFv-Cκ). Furthermore, crosslinking with HRP labeled mouse anti-Cκ antibodies improved M55 binding 10-fold (Kalinke et al., 2000; PMID: 10963674). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |