Mfg Part Number
Ab03918-1.1
Vector Labs Ab03918-1.1 Anti-M protein, serotype 5 [54.2.8], Mouse IgG1, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing BALB/c BYJ mice with streptococcal M type 5 membranes followed by a second, boosting dose of M type 5 streptococcal membranes.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 54.2.8 |
| Synonyms: | M protein, serotype 6; M protein, serotype 12; Myosin; Streptococcal M-protein; Keratin; DNA; Vimentin; Tropomyosin; Troponin; Ganglioside; Glycophorin M,N |
| Amount: | 100 μg |
| Application Codes Clone: | IF; WB; HA; PRNT; ELISA |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 11282394 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody reacts with α-helical molecules and is anti-nuclear (binds DNA). Specifically, it binds streptococcal M-protein (type 5), human myosin, keratin, DNA, vimentin, tropomyosin, troponin, ganglioside, and glycophorin M,N. |
| Application Notes: | This antibody reacts with rabbit myosin, as shown in a WB. This antibody reacts with M type 5 membrane proteins and human heart cell extract, as shown in an ELISA (Krisher et al., 1985; PMID: 2578225). This antibody reacts with the heavy chain of cardiac myosin. Specifically, it reacts with both heavy and light meromyosin fragments, as shown in WB and competitive inhibition assays (Cunningham et al., 1986; PMID: 3510006). This antibody stains the nuclei and cytoplasm of cells within the epidermis and epidermal appendages, as well as cells scattered throughout the dermis in normal and psoriatic skin, as shown by immunofluorescent staining (Swerlick et al., 1986; PMID: 3525693). This antibody binds CV (B3), as shown in an ELISA; neutralizes PV-1 and CV (B3), as shown in a plaque-reduction neutralization assay; and is moderately cytotoxic to rat fibroblasts, as shown in a cytotoxicity assay (Cunningham et al., 1992; PMID: 1311095). This antibody reacted with retinal S-antigen (S-Ag) in an ELISA (Lerner et al., 1995; PMID: 8722579). This antibody binds myosin at a high relative avidity, as determined in a competitive ELISA. Reactivity with glycophorin M and N was determined by hemagglutination assay (Mertens et al., 2000; PMID: 11282394). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |