Mfg Part Number
Ab03960-23.0
Vector Labs Ab03960-23.0 Anti-Insulin receptor [BBBB138], Rabbit IgG, kappa (100 μg)
Quick Overview
The original antibody was isolated from a human phage display library by panning against IR.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | BBBB138 |
| Synonyms: | IR |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; SPR; in vitro; in vivo |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | Fab |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | The antibody is specific for the insulin receptor. The antibody recognises both short isoform (IR-A) and a long isoform (IR-B). The epitope of the antibody does not overlap with the insulin binding site. |
| Application Notes: | The specificity of the antibody for IR was determined by ELISA analysis. The binding affinity of the antibody to IR-A and IR-B was measured by surface plasmon resonance (Kd= 2.57 and 2.98 nM respectively). The antibody blocked insulin induced IR phosphorylation in a dose-dependent manner on HuH7 cells. The antibody effectively decreased insulin induced glucose uptake at concentrations of 1 nM and 10 nM in vitro. The antibody induced hyperglycemia in C57 mice in in vivo experiments. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |