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Mfg Part Number

Ab03965-10.29

Vector Labs Ab03965-10.29 Anti-CD32b [NOV0281], Human Fab fragment, His-Tagged, lambda (100 μg)

VECTOR LABS

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Quick Overview

The original antibody was isolated from a human combinatorial antibody library by panning against biotinylated human CD32b.

$692.90

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Description

Product Class: Purified
Clone ID: NOV0281
Heavy Chain Modification: His-Tagged
Synonyms: CD32; Low affinity immunoglobulin gamma Fc region receptor II-b; IgG Fc receptor II-b; CDw32; Fc-gamma RII-b; Fc-gamma-RIIb; FcRII-b; human FCGR2B
Amount: 100 μg
Application Codes Clone: ELISA; FC; in vitro
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: Fab
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This reformatted human antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: The antibody specifically binds to CD32b. The antibody mainly recognizes epitope I (i.e., the Fc binding domain area). The antibody does not cross reacts with human CD32a-R.
Application Notes: The specificity of the antibody for CD32b was determined by ELISA analysis. The antibody was able to bind to CD32b expressed on human B cells by flow cytometry analysis (binding affinity 5.4 nM). The binding of the antibody to the BJAB cell line was evaluated by flow cytometry (binding affinity 2.4 nM). The antibody bound to CHO cells expressing huCD32b in flow cytometry analysis. The human IgG1 format of the antibody was evaluated for its activity in a primary NK cell based antibody-dependent cell-mediated cytotoxicity (ADCC) assay. The antibody demonstrated concentration dependent specific cell lysis of both cancer cell lines evaluated (Jeko-1 and Karpas422 Cancer Cell) (US20170198040A1).

Product Class:	Purified
Clone ID:	NOV0281
Heavy Chain Modification:	His-Tagged
Synonyms:	CD32; Low affinity immunoglobulin gamma Fc region receptor II-b; IgG Fc receptor II-b; CDw32; Fc-gamma RII-b; Fc-gamma-RIIb; FcRII-b; human FCGR2B
Amount:	100 μg
Application Codes Clone:	ELISA; FC; in vitro
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	Fab
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This reformatted human antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	The antibody specifically binds to CD32b. The antibody mainly recognizes epitope I (i.e., the Fc binding domain area). The antibody does not cross reacts with human CD32a-R.
Application Notes:	The specificity of the antibody for CD32b was determined by ELISA analysis. The antibody was able to bind to CD32b expressed on human B cells by flow cytometry analysis (binding affinity 5.4 nM). The binding of the antibody to the BJAB cell line was evaluated by flow cytometry (binding affinity 2.4 nM). The antibody bound to CHO cells expressing huCD32b in flow cytometry analysis. The human IgG1 format of the antibody was evaluated for its activity in a primary NK cell based antibody-dependent cell-mediated cytotoxicity (ADCC) assay. The antibody demonstrated concentration dependent specific cell lysis of both cancer cell lines evaluated (Jeko-1 and Karpas422 Cancer Cell) (US20170198040A1).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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