Mfg Part Number
Ab03983-10.0
Vector Labs Ab03983-10.0 Anti-von Willebrand factor [82D6A3], Human IgG1, kappa (100 μg)
Quick Overview
The original antibody was purified from murine ascites by Protein A chromatography.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | 82D6A3 |
| Synonyms: | human vWF A3 domain; VWF-A3; vWF |
| Amount: | 100 μg |
| Application Codes Clone: | Inhibition; in vivo; Crystallization |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for human vWF, A3 domain. |
| Application Notes: | This antibody was used as an inhibitor to study platelet adhesion to collagen VI and ECM. It was found to inhibit the binding of von Willebrand factor (VWF) to collagen I and III (Hoylaerts et al., 1997; PMID: 9164855). This antibody was found to have a comparable affinity for baboon vWF as for human vWF and inhibited the binding of baboon vWF to collagen with an inhibitory concentration of 50% (IC50) of 3.5 μg/ml. Furthermore, it prevented the binding of VWF to collagen and removed bound VWF from a collagen surface, albeit with a lower efficacy. Moreover, the antithrombotic efficacy of the antibody was evaluated in a modified Folts model in baboons; this antibody was used to inhibit the interaction between collagen and vWF to prevent arterial thrombosis in baboons (Wu et al., 2002; PMID: 11986216). The crystal structure of the antibody's Fab fragment, in complex with the vWF A3 domain, was determined (Staelens et al., 2006; PMID: 16314412). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |