Vector Labs Ab03987-23.0 Anti-AIF1 [GT1-mAb2], Rabbit IgG, lambda (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: GT1-mAb2
Synonyms: AIF1; IBA1; allograft inflammatory factor 1; AIF-1; IRT-1; interferon gamma responsive transcript; ionized calcium-binding adapter molecule 1; Daintain; DADB-70P7.8; allograft inflammatory factor-1 splice variant Hara-1; IRT1; protein G1
Amount: 100 μg
Application Codes Clone: ELISA; IF; FC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Goat IgG format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific to EBP05419, an immunizing peptide with the sequence C-TGPPAKKAISELP. This peptide was used in the production of the polyclonal Goat Anti-AIF1 antibody from which this antibody is derived.
Application Notes: This antibody (GT1-mAb2) was one of two monoclonal antibodies discovered by analyzing a polyclonal antibody (GT-1) sample. Briefly, de novo sequencing of GT-1 resulted in the full-length sequences of two distinct monoclonal antibodies (GT1-mAb1 and GT1-mAb2) in both framework and CDR regions. Both antibodies were recombinantly expressed by Absolute Antibody in HEK293 cells and tested in various assays. GT1-mAb2 was tested against the peptide antigen in an ELISA, and it showed activity comparable to that of the original polyclonal antibody. GT1-mAb2 was found to bind the target peptide in a non-competitive manner with GT1-mAb1 while being efficiently blocked by the original polyclonal antibody, proving that both monoclonal antibodies bind distinct epitopes and that GT1-mAb2 possess binding characteristics of the input polyclonal antibody. Furthermore, GT1-mAb2 was found to be compatible with immunofluorescence (IF) and flow cytometry (FC), although with an FC signal observedly less distinct than that of GT1-mAb1.

Product Class:	Purified
Clone ID:	GT1-mAb2
Synonyms:	AIF1; IBA1; allograft inflammatory factor 1; AIF-1; IRT-1; interferon gamma responsive transcript; ionized calcium-binding adapter molecule 1; Daintain; DADB-70P7.8; allograft inflammatory factor-1 splice variant Hara-1; IRT1; protein G1
Amount:	100 μg
Application Codes Clone:	ELISA; IF; FC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Goat IgG format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific to EBP05419, an immunizing peptide with the sequence C-TGPPAKKAISELP. This peptide was used in the production of the polyclonal Goat Anti-AIF1 antibody from which this antibody is derived.
Application Notes:	This antibody (GT1-mAb2) was one of two monoclonal antibodies discovered by analyzing a polyclonal antibody (GT-1) sample. Briefly, de novo sequencing of GT-1 resulted in the full-length sequences of two distinct monoclonal antibodies (GT1-mAb1 and GT1-mAb2) in both framework and CDR regions. Both antibodies were recombinantly expressed by Absolute Antibody in HEK293 cells and tested in various assays. GT1-mAb2 was tested against the peptide antigen in an ELISA, and it showed activity comparable to that of the original polyclonal antibody. GT1-mAb2 was found to bind the target peptide in a non-competitive manner with GT1-mAb1 while being efficiently blocked by the original polyclonal antibody, proving that both monoclonal antibodies bind distinct epitopes and that GT1-mAb2 possess binding characteristics of the input polyclonal antibody. Furthermore, GT1-mAb2 was found to be compatible with immunofluorescence (IF) and flow cytometry (FC), although with an FC signal observedly less distinct than that of GT1-mAb1.

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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