Vector Labs Ab03990-10.3 Anti-Gamma Delta TCR [G5-4], Human IgG1, Fc Silent™, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: G5-4
Heavy Chain Modification: Fc Silent™
Synonyms: TCR γδ; γδ TCR; TCR GD; GD TCR; TCRg/d; T-cell surface glycoprotein CD3 delta chain; CD3D; T3D; T-cell receptor T3 delta chain; T-cell surface glycoprotein CD3 gamma chain; CD3G; T-cell receptor T3 gamma chain; CD3g; T3G
Amount: 100 μg
Application Codes Clone: FC; functional assay
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2a
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for the human TCR delta and gamma chains. The T-cell receptor (TCR) is a molecule found on the surface of T cells, or T lymphocytes, that is responsible for recognizing fragments of antigen as peptides bound to major histocompatibility complex (MHC) molecules. On activation through antigen presenting cells (APCs), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. TCR part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response.
Application Notes: The binding of this antibody to γδ T cells was determined using flow cytometry. This antibody can compete with commercially available anti-γδ TCR antibody IMMU510 for binding to gamma-delta T cells. It is proposed that, since there is some competition between the two antibodies, G5-4 binds a similar or identical epitope to IMMU510. In an in vitro proliferation assay, this antibody was reported to positively stimulate the proliferation of γδT cells. The scFv version of this antibody was also reported to stimulate the secretion of cytokines like IFN-γ and TGF-α. G5-4ScFv-amplified γδT cells can effectively kill daudi cells, and the killing efficiency is greater than 85% when the effect-to-target ratio is 5:1 (CN103130894).

Product Class:	Purified
Clone ID:	G5-4
Heavy Chain Modification:	Fc Silent™
Synonyms:	TCR γδ; γδ TCR; TCR GD; GD TCR; TCRg/d; T-cell surface glycoprotein CD3 delta chain; CD3D; T3D; T-cell receptor T3 delta chain; T-cell surface glycoprotein CD3 gamma chain; CD3G; T-cell receptor T3 gamma chain; CD3g; T3G
Amount:	100 μg
Application Codes Clone:	FC; functional assay
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2a
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for the human TCR delta and gamma chains. The T-cell receptor (TCR) is a molecule found on the surface of T cells, or T lymphocytes, that is responsible for recognizing fragments of antigen as peptides bound to major histocompatibility complex (MHC) molecules. On activation through antigen presenting cells (APCs), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. TCR part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response.
Application Notes:	The binding of this antibody to γδ T cells was determined using flow cytometry. This antibody can compete with commercially available anti-γδ TCR antibody IMMU510 for binding to gamma-delta T cells. It is proposed that, since there is some competition between the two antibodies, G5-4 binds a similar or identical epitope to IMMU510. In an in vitro proliferation assay, this antibody was reported to positively stimulate the proliferation of γδT cells. The scFv version of this antibody was also reported to stimulate the secretion of cytokines like IFN-γ and TGF-α. G5-4ScFv-amplified γδT cells can effectively kill daudi cells, and the killing efficiency is greater than 85% when the effect-to-target ratio is 5:1 (CN103130894).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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