Mfg Part Number
Ab03993-30.11

Vector Labs Ab03993-30.11 Anti-Tetracycline [scFvTc], scFv fragment (His), ScFv, (50 μg)

Quick Overview
The original antibody was generated by immunizing BALB/c mice with tetracycline hydrochloride-BSA as an immunogen. The cDNA obtained from total mRNA of the immunized mouse splenocytes was used as a template to amplify the variable domain genes and generate an scFv phage display library. Later on the antibody was selected by panning this library against tetracycline-ovalbumin conjugate.
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Product Class:Purified
Clone ID:scFvTc
Heavy Chain Modification:ScFv
Synonyms:Tc; antibiotic; tetracycline hydrochloride; aureomycin; oxytetracycline; doxycycline
Amount:50 μg
Application Codes Clone:ELISA
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:scFv
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:This antibody binds tetracycline. Tetracycline, is an antibiotic derived from microorganisms of the genus Streptomyces and are broadly used to treat bacterial infections. This antibody is reported to cross-react with aureomycin and also shows weak reactivity for oxytetracycline and doxycycline.
Application Notes:The binding characterization of this antibody for tetracycline was done using ELISA. Additionally, this antibody was also capable of cross-reacting with aureomycin, oxytetracycline and doxycycline in an ELISA. The colloidal gold labelled version of this antibody was used for the development of colloidal gold test strip for detection of tetracycline hydrochloride (CN103396484).
More Information
Brand Vector Labs
UNSPSC 12161500