Mfg Part Number
Ab03999-1.1
Vector Labs Ab03999-1.1 Anti-Tritrpticin [6C6D7], Mouse IgG1, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing female BALB/c mice of 6-8 weeks of age with 200 µg of K-tritrpticin-diphtheria toxoid conjugate emulsified with equal volume of complete Freund’s adjuvant.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 6C6D7 |
| Synonyms: | C6; Prophenin and tritrpticin precursor |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 24086578 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody binds tritrpticin (VRRFPWWWPFLRR), which is a member of the cathelicidin family, a 13mer tryptophan-rich group of diverse cationic antimicrobial peptides found in neutrophil granules. It has a broad spectrum activity against a range of microorganisms, fungi, and protozoa. |
| Application Notes: | The binding characterization of this antibody was done using ELISA. In an indirect-binding ELISA where the peptides were directly immobilized on to the ELISA plate, mAb 6C6D7 could bind to tritrpticin and its N-terminal deleted antibacterial analogs at nanomolar concentrations. The binding affinity of this antibody to K-tritrpticin was analyzed using surface plasmon resonance and it was reported to be Kd= 350 nM. The binding of 6C6D7 to tritrpticin is characterized by negligible change in ΔGeq with respect to temperature that suggests that the antibody does not undergo any major conformational alteration during the binding of peptide antigen. This antibody was also used to bind tritripticin and related peptides to further analyze their anti-microbial activity (PMID: 24086578). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |