JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser.

Skip to the beginning of the images gallery

Mfg Part Number

Ab04008-30.11

Vector Labs Ab04008-30.11 Anti-Factor VIII [KM33], scFv fragment (His), ScFv, (50 μg)

VECTOR LABS

Be the first to review this product

Quick Overview

The original antibody was isolated from a human phage display library generated from the peripheral blood of an inhibitor patient and panning the library against factor VIII light chain.

$692.90

Order will ship within 1-3 business days

Description

Product Class: Purified
Clone ID: KM33
Heavy Chain Modification: ScFv
Synonyms: AHF; FVIII; Factor 8; anti-hemophilic factor; Coagulation factor VIII; procoagulant component
Amount: 50 μg
Application Codes Clone: ELISA; inhibit; functional assay
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: scFv
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody binds the C1 domain of the human factor VIII, a glycoprotein involved in blood coagulation. Factor VIII acts as a cofactor for factor IXa which converts factor X to the activated form Xa in the presence of calcium and phospholipids. Defects in the F8 gene, which encodes Factor VIII, result in the coagulation disorder hemophilia A.
Application Notes: The scFv version of this antibody was capable of inhibiting factor VIII activity with inhibition titre of 97 BU/mg. The scFv antibody binds human factor VIII light chain with a binding affinity of Kd= 0.1 nM. It was reported that scFv KM33 inhibits the procoagulant activity of factor VIII, although its epitope is located outside region R1803-K1818 (PMID: 11159524). It was reported that the presence of this antibody completely inhibited FVIII endocytosis by both monocyte and bone marrow derived mouse dendritic cells (PMID: 21962992). It was reported that this antibody inhibits FVIII activity and interactions with its physiological ligands, von Willebrand factor, phospholipids and catabolic receptors in vitro (PMID: 11159524; Limburg V et al., 2005).

Product Class:	Purified
Clone ID:	KM33
Heavy Chain Modification:	ScFv
Synonyms:	AHF; FVIII; Factor 8; anti-hemophilic factor; Coagulation factor VIII; procoagulant component
Amount:	50 μg
Application Codes Clone:	ELISA; inhibit; functional assay
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	scFv
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody binds the C1 domain of the human factor VIII, a glycoprotein involved in blood coagulation. Factor VIII acts as a cofactor for factor IXa which converts factor X to the activated form Xa in the presence of calcium and phospholipids. Defects in the F8 gene, which encodes Factor VIII, result in the coagulation disorder hemophilia A.
Application Notes:	The scFv version of this antibody was capable of inhibiting factor VIII activity with inhibition titre of 97 BU/mg. The scFv antibody binds human factor VIII light chain with a binding affinity of Kd= 0.1 nM. It was reported that scFv KM33 inhibits the procoagulant activity of factor VIII, although its epitope is located outside region R1803-K1818 (PMID: 11159524). It was reported that the presence of this antibody completely inhibited FVIII endocytosis by both monocyte and bone marrow derived mouse dendritic cells (PMID: 21962992). It was reported that this antibody inhibits FVIII activity and interactions with its physiological ligands, von Willebrand factor, phospholipids and catabolic receptors in vitro (PMID: 11159524; Limburg V et al., 2005).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

Reviews