JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser.

Skip to the beginning of the images gallery

Mfg Part Number

Ab04009-1.1

Vector Labs Ab04009-1.1 Anti-Factor VIII [KM41], Mouse IgG1, kappa (100 μg)

VECTOR LABS

Be the first to review this product

Quick Overview

The original antibody was isolated from a human phage display library generated from the peripheral blood of an inhibitor patient and panning the library against factor VIII light chain.

$570.70

Order will ship within 1-3 business days

Description

Product Class: Purified
Clone ID: KM41
Synonyms: AHF; FVIII; Factor 8; anti-hemophilic factor; Coagulation factor VIII; procoagulant component
Amount: 100 μg
Application Codes Clone: ELISA; inhibit; functional assay
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: scFv
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This full-length, chimeric mouse antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds and epitope between amino acid R1803-K1818 of the A3-C1 domains of the human factor VIII, which also contain binding site for factor IXa. Factor VIII is a glycoprotein involved in blood coagulation. Factor VIII acts as a cofactor for factor IXa which converts factor X to the activated form Xa in the presence of calcium and phospholipids. Defects in the F8 gene, which encodes Factor VIII, result in the coagulation disorder hemophilia A.
Application Notes: The scFv version of this antibody was capable of inhibiting factor VIII activity with inhibition titre of 63 BU/mg. The scFv antibody binds human factor VIII light chain with a binding affinity of Kd= 1.6 nM (PMID: 11159524). It was reported that scFv KM33 inhibits the procoagulant activity of factor VIII. In view of the similar epitope specificity of scFv KM41 and CLB-CAg A (antibody which binds a synthetic peptide comprising residues R1803-K1818), it is likely that scFv KM41 competes with factor IXa for binding to the factor VIII light chain (PMID: 11159524).

Product Class:	Purified
Clone ID:	KM41
Synonyms:	AHF; FVIII; Factor 8; anti-hemophilic factor; Coagulation factor VIII; procoagulant component
Amount:	100 μg
Application Codes Clone:	ELISA; inhibit; functional assay
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	scFv
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This full-length, chimeric mouse antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds and epitope between amino acid R1803-K1818 of the A3-C1 domains of the human factor VIII, which also contain binding site for factor IXa. Factor VIII is a glycoprotein involved in blood coagulation. Factor VIII acts as a cofactor for factor IXa which converts factor X to the activated form Xa in the presence of calcium and phospholipids. Defects in the F8 gene, which encodes Factor VIII, result in the coagulation disorder hemophilia A.
Application Notes:	The scFv version of this antibody was capable of inhibiting factor VIII activity with inhibition titre of 63 BU/mg. The scFv antibody binds human factor VIII light chain with a binding affinity of Kd= 1.6 nM (PMID: 11159524). It was reported that scFv KM33 inhibits the procoagulant activity of factor VIII. In view of the similar epitope specificity of scFv KM41 and CLB-CAg A (antibody which binds a synthetic peptide comprising residues R1803-K1818), it is likely that scFv KM41 competes with factor IXa for binding to the factor VIII light chain (PMID: 11159524).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

Reviews