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Mfg Part Number

Ab04011-10.3

Vector Labs Ab04011-10.3 Anti-Factor VIII [EL-14], Human IgG1, Fc Silent™, kappa (100 μg)

VECTOR LABS

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Quick Overview

The original antibody was isolated from a human phage display library generated from the peripheral blood of an inhibitor patient and panning the library against factor VIII light chain.

$570.70

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Description

Product Class: Purified
Clone ID: EL-14
Heavy Chain Modification: Fc Silent™
Synonyms: AHF; FVIII; Factor 8; anti-hemophilic factor; Coagulation factor VIII; procoagulant component
Amount: 100 μg
Application Codes Clone: IP; inhibit; functional assay
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: scFv
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This full-length, reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds the C2 domain of the human factor VIII, a glycoprotein involved in blood coagulation. Factor VIII acts as a cofactor for factor IXa which converts factor X to the activated form Xa in the presence of calcium and phospholipids. Defects in the F8 gene, which encodes Factor VIII, result in the coagulation disorder hemophilia A.
Application Notes: The binding characterization of this antibody towards radiolabeled FVIII light chain and the C2 domain of factor VIII was done using immunoprecipitation. The inhibitory activity of this antibody against factor VIII was determined using a one-stage clotting assay. This antibody was capable of completely eliminating FVIII inhibition by CLB-CAg 117, a C2 domain directed anti-factor VIII antibody (PMID: 10627462). The scFv version of this antibody bound factor VIII light chain with a dissociation constant of Kd = 1.1 nM (PMID: 11929772).

Product Class:	Purified
Clone ID:	EL-14
Heavy Chain Modification:	Fc Silent™
Synonyms:	AHF; FVIII; Factor 8; anti-hemophilic factor; Coagulation factor VIII; procoagulant component
Amount:	100 μg
Application Codes Clone:	IP; inhibit; functional assay
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	scFv
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This full-length, reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds the C2 domain of the human factor VIII, a glycoprotein involved in blood coagulation. Factor VIII acts as a cofactor for factor IXa which converts factor X to the activated form Xa in the presence of calcium and phospholipids. Defects in the F8 gene, which encodes Factor VIII, result in the coagulation disorder hemophilia A.
Application Notes:	The binding characterization of this antibody towards radiolabeled FVIII light chain and the C2 domain of factor VIII was done using immunoprecipitation. The inhibitory activity of this antibody against factor VIII was determined using a one-stage clotting assay. This antibody was capable of completely eliminating FVIII inhibition by CLB-CAg 117, a C2 domain directed anti-factor VIII antibody (PMID: 10627462). The scFv version of this antibody bound factor VIII light chain with a dissociation constant of Kd = 1.1 nM (PMID: 11929772).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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