Mfg Part Number
Ab04023-21.0
Vector Labs Ab04023-21.0 Anti-Podocalyxin [mAb4F10], Mouse IgM, kappa (50 μg)
Quick Overview
The original antibody was generated by immunizing BALB/C mice with purified human podocalyxin-glutathione S-transferase (GST) fusion protein containing the intracellular, transmembrane, and a part of the extracellular domain of human PCLP.
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| Product Class: | Purified |
|---|---|
| Clone ID: | mAb4F10 |
| Synonyms: | PC; PCLP-1; GCTM-2 antigen; Gp200; Podocalyxin-like protein 1; 4F10 |
| Amount: | 50 μg |
| Application Codes Clone: | IF; WB; IP |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgM |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | The antibody is specific for human podocalyxin. The epitope recognized by the antibody is located on the intracellular domain of podocalyxin. Podocalyxin, a sialoglycoprotein, is thought to be the major constituent of the glycocalyx of podocytes in the glomerulus (Bowman's capsule) in the kidneys. It is a member of the CD34 family of transmembrane sialomucins. |
| Application Notes: | The antibody recognized rabbit PCLP1 in human renal cortical sections on immunofluorescence. The antibody detected PCLP by western blot analysis. The antibody recognized and immunoprecipitated rat podocalyxin (Kershaw et al., 1997; PMID: 9188463). The antibody detected PCLP in HEV of tonsil by immunofluorescence analysis (Sassetti et al., 1998; PMID: 9625756). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |