Vector Labs Ab04037-10.3 Anti-Occludin [37-5], Human IgG1, Fc Silent™, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 37-5
Heavy Chain Modification: Fc Silent™
Synonyms: OCLN
Amount: 100 μg
Application Codes Clone: FC; ELISA; TER; ICC; Blocking; Inhibition; in vitro; in vivo; IP; Immunoblot
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2b
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for the first extracellular loop (EL1) of OCLN.
Application Notes: The cell binding capability of the original format of this antibody (rat IgG2b) was assessed using FC, and its binding to HT1080/hOCLN cells was assessed using a cell-based ELISA. This antibody was found to have no negative effect on tight junction (TJ) barrier function, as determined by a TJ barrier function assay (TER). Furthermore, this antibody was found to have no negative effect on cell viability, as determined by a colorimetric XTT assay. This antibody was found to strongly inhibit (in vitro) and fully block (in vivo) HCV infections, as measured by ICC (Shimizu et al., 2018; PMID: 29437969). This antibody's K_d for hOCLN is 0.720 ± 0.084 nM, as measured in a cell-based competition ELISA. This antibody successfully precipitated FLAG-hOCLN proteins found in HT1080 cell lysates and endogenously expressed cellular OCLN found in Caco-2, HepG2, and MCF-7 cell lysates. Furthermore, other TJ proteins expressed in T84 cells — TRIC and ZO-2 — were found to be co-immunoprecipitated with OCLN by this antibody. This antibody can be used for immunoblot analysis of OCLN (only at high concentrations due to a weak response). Immunofluorescence (IF) staining of OCLN using clone 1-3 with a TJ marker protein ZO-1 in Caco-2 cells was co-localized with ZO-1 at cell-cell contact sites. This antibody cross-reacts with crab-eating monkey OCLN (moOCLN), as determined by FC (Shimizu et al., 2019; PMID: 31077306).

Product Class:	Purified
Clone ID:	37-5
Heavy Chain Modification:	Fc Silent™
Synonyms:	OCLN
Amount:	100 μg
Application Codes Clone:	FC; ELISA; TER; ICC; Blocking; Inhibition; in vitro; in vivo; IP; Immunoblot
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2b
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for the first extracellular loop (EL1) of OCLN.
Application Notes:	The cell binding capability of the original format of this antibody (rat IgG2b) was assessed using FC, and its binding to HT1080/hOCLN cells was assessed using a cell-based ELISA. This antibody was found to have no negative effect on tight junction (TJ) barrier function, as determined by a TJ barrier function assay (TER). Furthermore, this antibody was found to have no negative effect on cell viability, as determined by a colorimetric XTT assay. This antibody was found to strongly inhibit (in vitro) and fully block (in vivo) HCV infections, as measured by ICC (Shimizu et al., 2018; PMID: 29437969). This antibody's K_d for hOCLN is 0.720 ± 0.084 nM, as measured in a cell-based competition ELISA. This antibody successfully precipitated FLAG-hOCLN proteins found in HT1080 cell lysates and endogenously expressed cellular OCLN found in Caco-2, HepG2, and MCF-7 cell lysates. Furthermore, other TJ proteins expressed in T84 cells — TRIC and ZO-2 — were found to be co-immunoprecipitated with OCLN by this antibody. This antibody can be used for immunoblot analysis of OCLN (only at high concentrations due to a weak response). Immunofluorescence (IF) staining of OCLN using clone 1-3 with a TJ marker protein ZO-1 in Caco-2 cells was co-localized with ZO-1 at cell-cell contact sites. This antibody cross-reacts with crab-eating monkey OCLN (moOCLN), as determined by FC (Shimizu et al., 2019; PMID: 31077306).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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