Mfg Part Number
Ab04038-23.0
Vector Labs Ab04038-23.0 Anti-Occludin [67-2], Rabbit IgG, lambda (100 μg)
Quick Overview
The original antibody was generated by immunizing mice with KLH-coupled, modified hOCLN-EC2 peptide. The modification made to the hOCLN-EC2 peptide was an added cysteine residue was at the N-terminal end.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 67-2 |
| Synonyms: | OCLN |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; IF; Inhibition; in vitro; Immunoblot |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for the peptide ALCNQFYTPAATGLYVD, which is part of the second extracellular loop (EL2) of human OCLN (aa 214-230). However, it has also been shown to bind mouse OCLN, strongly suggesting that it does not recognize the human-specific amino acids in OCLN-EC2. |
| Application Notes: | The original format of this antibody (mouse IgG1) can bind hOCLN, as was determined by ELISA during initial hybridoma screening. This antibody efficiently inhibited HCV infection in the HCV-permissive human cell line Huh7.5.1. This antibody can access OCLN from the basolateral side of hepatocytes but not from the apical side using three different culture systems of Huh7.5.1 cells. This antibody was shown to cross-react with mouse OCLN in a WB. This antibody showed no adverse effects on cell viability or the barrier function of tight junctions (Okai et al., 2018; PMID: 29682171). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |