Mfg Part Number
Ab04039-10.3
Vector Labs Ab04039-10.3 Anti-S. aureus β-GlcNAc WTA [4497], Human IgG1, Fc Silent™, kappa (100 μg)
Quick Overview
The original antibody was generated by cloning human IgG antibodies from peripheral B cells of patients who had been infected with S. aureus.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 4497 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | TA; Teichoic acids; WTA; Wall teichoic acids; GlcNAc; N-Acetylglucosamine; β-WTA; β-GlcNAc; β-1,4-GlcNAc; β-O-linked N-acetylglucosamine; β-O-linked GlcNAc sugar modifications on WTA |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; FC; RBA; IF; In vitro; In vivo |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody is specific for S. aureus β-GlcNAc wall-teichoic acids (WTAs), a pathogen-specific polyanionic glycopolymer that is connected to the thick peptidoglycan layers of Gram-positive bacteria. |
| Application Notes: | The original format of this antibody (human IgG1) was screened for antigen binding specificity using ELISA, and it was successfully used antibody was used in flow cytometry (FC) to label S. aureus (Lehar et al., 2015; PMID: 26536114). This antibody was used in a radioligand binding assay (RBA); affinity analysis and epitope counting were performed by saturation binding analysis using a radio-iodinated version of the antibody (Fong et al., 2018; PMID: 30102105). This antibody was observed to bind strongly to planktonic Wood46 (ATCC 10832) S. aureus cells but very weakly to planktonic LAC∆spa∆sbi (AH4116) S. aureus. When studying the binding of WTA-specific antibodies to biofilms, this antibody was observed to bind strongly to PNAG-positive biofilm formed by Wood46 and bind S. aureus Wood46 in both planktonic and biofilm states. Apart from recognizing PNAG-positive biofilms, this antibody was also found to bind the PNAG-negative biofilm formed by LAC∆spa∆sbi. Using IF, this antibody was confirmed to bind WTA in PNAG-positive and PNAG-negative in vitro biofilm. This antibody was tested in a subcutaneously implanted pre-colonized catheter in mice and was found to localize to biofilm rapidly in vivo (de Vor et al., 2022; PMID: 34989676). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |