JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser.

Skip to the beginning of the images gallery

Mfg Part Number

Ab04040-3.0

Vector Labs Ab04040-3.0 Anti-Cytochrome c [2.7D5], Mouse IgG2b, kappa (100 μg)

VECTOR LABS

Be the first to review this product

Quick Overview

The original antibody — a secondary immune response antibody — was generated by immunizing BALB/c mice with pigeon cytochrome C covalently coupled to ovalbumin using glutaraldehyde (CYT-OVA) and later challenging them with the same immunogen (pigeon CYT-OVA).

$570.70

Order will ship within 1-3 business days

Description

Product Class: Purified
Clone ID: 2.7D5
Synonyms: CYCS; CYC; HCS; THC4; Cytochrome c, somatic; Cytochrome c; Cyt c
Amount: 100 μg
Application Codes Clone: ELISA; IF; IP; Immunogold labeling
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2b
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody is specific for pigeon cytochrome c (CYT) and cross-reacts with mouse CYT. Specifically, it recognizes the region around residue 62 of native mouse CYT.
Application Notes: The binding specificity of the original format of this antibody (mouse IgG2b) was evaluated by indirect ELISA, and its binding affinity was determined by competitive ELISA. It was found to bind Pigeon CYT ~5.6 times better than Mouse CYT (Liu et al., 2000; PMID: 11257306). This antibody was used in the detection of intracellular mouse cytochrome c (cyt c) in live DO11.10 T hybridoma cells by immunofluorescent (IF) labeling. Specifically, it was found to recognize the surface around residue 62 of cyt c and bound to cyt c in fixed and permeabilized cells. Furthermore, it was used in indirect IF staining of cyt c in post-apoptotic cells and immunoprecipitation (IP) of cyt c extracted from live, apoptotic, and necrotic DO11.10 cells (Jemmerson et al., 1999; PMID: 10090746). This antibody was used in immunogold labeling to determine the subcellular localization of cytochrome-c in rat pancreatic acinar cells (Soltys et al., 2001; PMID: 11319839).

Product Class:	Purified
Clone ID:	2.7D5
Synonyms:	CYCS; CYC; HCS; THC4; Cytochrome c, somatic; Cytochrome c; Cyt c
Amount:	100 μg
Application Codes Clone:	ELISA; IF; IP; Immunogold labeling
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2b
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody is specific for pigeon cytochrome c (CYT) and cross-reacts with mouse CYT. Specifically, it recognizes the region around residue 62 of native mouse CYT.
Application Notes:	The binding specificity of the original format of this antibody (mouse IgG2b) was evaluated by indirect ELISA, and its binding affinity was determined by competitive ELISA. It was found to bind Pigeon CYT ~5.6 times better than Mouse CYT (Liu et al., 2000; PMID: 11257306). This antibody was used in the detection of intracellular mouse cytochrome c (cyt c) in live DO11.10 T hybridoma cells by immunofluorescent (IF) labeling. Specifically, it was found to recognize the surface around residue 62 of cyt c and bound to cyt c in fixed and permeabilized cells. Furthermore, it was used in indirect IF staining of cyt c in post-apoptotic cells and immunoprecipitation (IP) of cyt c extracted from live, apoptotic, and necrotic DO11.10 cells (Jemmerson et al., 1999; PMID: 10090746). This antibody was used in immunogold labeling to determine the subcellular localization of cytochrome-c in rat pancreatic acinar cells (Soltys et al., 2001; PMID: 11319839).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

Reviews