Mfg Part Number
Ab04079-34.11
Vector Labs Ab04079-34.11 Anti-Fibronectin EIIIB [NJB2], Camelid VHH, His-tagged, (100 μg)
Quick Overview
The original antibody was isolated from an alpaca-derived phage display library, by panning against the EIIIB domain of fibronectin.
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| Product Class: | Purified |
|---|---|
| Clone ID: | NJB2 |
| Heavy Chain Modification: | His-tagged |
| Synonyms: | FN-EIIIB; EDB domain of fibronectin |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; IF; IHC; Immunoblot; in vivo |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody is specific for the FN-EIIIB splice variant expressed in the ECM of tumors, metastases, and other diseases characterized by ECM deposition. |
| Application Notes: | This nanobody was shown to detect primary tumors and metastatic sites in vivo by specifically binding to the ECM with excellent specificity in multiple models of breast cancer, including human and mouse triple-negative breast cancer, and in melanoma, as measured by PET/CT imaging with 64Cu-NJB2. This nanobody recognized EIIIB-His by ELISA and in FN-containing EIIIB immunohistochemistry (IHC). Furthermore, its binding affinity to immobilized single EIIIB domain and a large recombinant rat FN fragment comprising FNIII7–15, including EIIIB (FN 7–15 EIIIB), was 1.9 × 10-9 M and 2.25 × 10-9 M, respectively, as determined by biolayer interferometry (BLI) (Jailkhani et al., 2019; PMID: 31068469). This nanobody was used for IHC of B16F10 and MC38 tumors to detect EIIIB expression (Xie et al., 2019; PMID: 30936321). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |