Mfg Part Number
Ab04081-23.159
Vector Labs Ab04081-23.159 Anti-GFP [GBP1 (Enh)], Rabbit IgG-Fc Fusion, (100 μg)
Quick Overview
The original antibody was generated by immunizing an alpaca with GFP.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | GBP1 (Enh) |
| Synonyms: | Green fluorescent protein; GFP Binding Protein 1; Enhancer |
| Amount: | 100 μg |
| Application Codes Clone: | IF; in vitro; in vivo |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Alpaca VHH format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for GFP. |
| Application Notes: | The Enhancer (Enh) anti-GFP nanobody, also known as GFP binding protein 1 (GBP1), was specifically used for manipulating protein conformation in vitro and in vivo; this nanobody caused a fourfold fluorescence enhancement by stabilizing the deprotonated, negatively charged state of the GFP chromophore, resulting in increased fluorescence intensity (Kirchhofer et al., 2010; PMID: 20010839). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |