Vector Labs Ab04088-10.0 Anti-Cathepsin B [2A2], Human IgG1, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 2A2
Synonyms: Cat B; CTSB; CPSB; APPS; APP secretase; Cathepsin B1
Amount: 100 μg
Application Codes Clone: inhibit; ELISA; WB
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds human cathepsin B (Cat B) at an epitope near the active site and inhibits its proteolytic activity. It was reported to specifically bind the 'EPGYSP' sequence located between amino acid residues 133–138 of cathepsin B. Cat B is a lysosomal cysteine protease that is shown to participate in processes of tumor growth, invasion, and metastasis. It is seen that the increased level of tumor cathepsin B is not balanced by an increase in cysteine proteases, leading to uncontrolled proteolysis in the extracellular matrix. Significantly increased levels of Cat B have also been found in sera of patients with breast, colorectal, liver, pancreatic, and melanoma cancers.
Application Notes: This antibody is capable of binding cathepsin B in an antigen-immobilized ELISA. The binding reactivity of this antibody to cathepsin B was also confirmed using Western blot. This antibody significantly inhibited the invasion of ras-transformed MCF-10A neoT cells through matrigel. This antibody was further reported to inhibit cathepsin B activity against BODYPY FL casein significantly (Fan et al., 2002; PMID: 12530548). In a study, the intracellular and extracellular cathepsin B activities in the invasion of ras-transformed human breast epithelial cells, MCF-10A neoT, were assessed using this antibody. This antibody was shown to impair both the intracellular and the extracellular fractions of cathepsin B activity (Premzl et al., 2003; PMID: 12581740). This antibody was also used in the generation of an ammodytoxin-anti-cathepsin B immuno-conjugate to deliver secretory phospholipase A2 into cancerous cells (Premzl et al., 2008; PMID: 18221975). This antibody was reported to potentiate cathepsin B exopeptidase activity with an activation constant (K_a) of 22.3 nm, although simultaneously inhibiting its endopeptidase activity (Mirković et al., 2009; PMID: 19656187).

Product Class:	Purified
Clone ID:	2A2
Synonyms:	Cat B; CTSB; CPSB; APPS; APP secretase; Cathepsin B1
Amount:	100 μg
Application Codes Clone:	inhibit; ELISA; WB
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds human cathepsin B (Cat B) at an epitope near the active site and inhibits its proteolytic activity. It was reported to specifically bind the 'EPGYSP' sequence located between amino acid residues 133–138 of cathepsin B. Cat B is a lysosomal cysteine protease that is shown to participate in processes of tumor growth, invasion, and metastasis. It is seen that the increased level of tumor cathepsin B is not balanced by an increase in cysteine proteases, leading to uncontrolled proteolysis in the extracellular matrix. Significantly increased levels of Cat B have also been found in sera of patients with breast, colorectal, liver, pancreatic, and melanoma cancers.
Application Notes:	This antibody is capable of binding cathepsin B in an antigen-immobilized ELISA. The binding reactivity of this antibody to cathepsin B was also confirmed using Western blot. This antibody significantly inhibited the invasion of ras-transformed MCF-10A neoT cells through matrigel. This antibody was further reported to inhibit cathepsin B activity against BODYPY FL casein significantly (Fan et al., 2002; PMID: 12530548). In a study, the intracellular and extracellular cathepsin B activities in the invasion of ras-transformed human breast epithelial cells, MCF-10A neoT, were assessed using this antibody. This antibody was shown to impair both the intracellular and the extracellular fractions of cathepsin B activity (Premzl et al., 2003; PMID: 12581740). This antibody was also used in the generation of an ammodytoxin-anti-cathepsin B immuno-conjugate to deliver secretory phospholipase A2 into cancerous cells (Premzl et al., 2008; PMID: 18221975). This antibody was reported to potentiate cathepsin B exopeptidase activity with an activation constant (K_a) of 22.3 nm, although simultaneously inhibiting its endopeptidase activity (Mirković et al., 2009; PMID: 19656187).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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