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Mfg Part Number

Ab04101-10.3

Vector Labs Ab04101-10.3 Anti-PLC-gamma phosphorylated [AbAb1-PLCy], Human IgG1, Fc Silent™, kappa (100 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by immunizing rabbits with recombinant phosphorylated peptide pPLC-γ(RNPGF-pY-VEANPGGGSC) conjugated with KLH as an immunogen.

$570.70

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Description

Product Class: Purified
Clone ID: AbAb1-PLCy
Heavy Chain Modification: Fc Silent™
Synonyms: PLCG1; PLC1; PLC-gamma-1; PLC-γ; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; PLC-148; Phosphoinositide phospholipase C-gamma-1; Phospholipase C-II; PLC-II; Phospholipase C-gamma-1
Amount: 100 μg
Application Codes Clone: ELISA; WB; IP
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Rabbit IgG format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds phosphorylated phosphoinositide phospholipase C-gamma, which mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). It also plays an important role in the regulation of intracellular signaling cascades.
Application Notes: The binding reactivity of this antibody to recombinant phosphorylated pPLC-γ peptide was confirmed using ELISA. The binding reactivity was further confirmed using western blot, of phosphorylated PLC-γ from NIH3T3 cell lysates. The scFv version of this antibody was used fused to human IgG1 using a suitable linker for the generation of scFv-Fc mini-antibody. This minibody was capable of immunoprecipitating pPLC-γ. The fluorescent scFv version of this antibody, when expressed intracellularly was reported to inhibit signal transduction by PLC-γ in transfected into NIH3T3 cells as observed using fluorescent microscopy (US20090233358).

Product Class:	Purified
Clone ID:	AbAb1-PLCy
Heavy Chain Modification:	Fc Silent™
Synonyms:	PLCG1; PLC1; PLC-gamma-1; PLC-γ; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; PLC-148; Phosphoinositide phospholipase C-gamma-1; Phospholipase C-II; PLC-II; Phospholipase C-gamma-1
Amount:	100 μg
Application Codes Clone:	ELISA; WB; IP
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Rabbit IgG format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds phosphorylated phosphoinositide phospholipase C-gamma, which mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). It also plays an important role in the regulation of intracellular signaling cascades.
Application Notes:	The binding reactivity of this antibody to recombinant phosphorylated pPLC-γ peptide was confirmed using ELISA. The binding reactivity was further confirmed using western blot, of phosphorylated PLC-γ from NIH3T3 cell lysates. The scFv version of this antibody was used fused to human IgG1 using a suitable linker for the generation of scFv-Fc mini-antibody. This minibody was capable of immunoprecipitating pPLC-γ. The fluorescent scFv version of this antibody, when expressed intracellularly was reported to inhibit signal transduction by PLC-γ in transfected into NIH3T3 cells as observed using fluorescent microscopy (US20090233358).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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