Mfg Part Number
Ab04106-1.1
Vector Labs Ab04106-1.1 Anti-Viral hemorrhagic septicemia virus [3F1A2], Mouse IgG1, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing BALB/c mice with Egtved virus by intraperitoneal injections five times over two months, followed by an additional intravenous booster injection on week nine. The hybridoma cell line secreting clone 3F1A2 was selected from a subsequent fusion after three more months of continued immunization.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 3F1A2 |
| Synonyms: | G; Spike glycoprotein |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; PNT; in vitro |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 10938729 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody is specific for the transmembrane envelope glycoprotein (G protein) of viral hemorrhagic septicemia virus (VHSV). The G protein is the only protein known to be present on the surface of the virus particle, and it constitutes less than 10% of the mass of the virus particle which is dominated by N, M1 and M2. This antibody demonstrated similar neutralizing activity to clone 3F1H10, and they were found to interfere with each other's binding reciprocally, which indicates they recognize the same or partially related epitopes. |
| Application Notes: | The neutralizing activity of the original format of this antibody (mouse IgG1) against different VHSV isolates was determined by 50% PNT; it neutralized type I isolates VHSV (I-F1 and I-92) and type II isolate VHSV (II-31) well, but higher concentrations were needed to neutralize type III isolates (III-51 and III-37). ELISA was used to detect and quantify the antibody's binding efficiency to various VHSV strains. The antibody's binding kinetics were evaluated by surface plasmon resonance (SPR) analysis resulting in a KD of 2.4 nM for the immobilized G protein of VHSV strain I-F1 and a KD of 12.1 nM for the immobilized G protein of VHSV strain I-92 (Lorenzen et al., 2000; PMID: 10938729). The Fab and recombinant single-chain antibody fragments (scAbs) versions of this antibody neutralized VHSV, indicating that the Fc moiety and divalency of the antibody molecules are unnecessary for neutralization. However, the neutralizing activity of Fab and scAb fragments was generally lower compared to the parent antibody due to the higher avidity of the divalent parent molecules; the Fab and scAb fragments exhibited a 10-100-fold increase in kd compared to the parent MAb, while the kas were similar. The increased kd was accompanied by decreased neutralizing activity. The KD measured against immobilized G protein of VHSV strain DK-F1 was 2.4 nM for the parent MAb and 227 and 77 nM for the daughter Fab and scAb, respectively (Cupit et al., 2001; PMID: 11682124). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |