Vector Labs Ab04107-1.1 Anti-IgG [RF61], Mouse IgG1, lambda (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: RF61
Synonyms: IgG1; IgG2; IgG3; IgG4; Immunoglobulin G; Immunoglobulin gamma-1; Immunoglobulin G subclass 1; Immunoglobulin gamma-2; Immunoglobulin G subclass 2; Immunoglobulin gamma-3; Immunoglobulin G subclass 3; Immunoglobulin gamma-4; Immunoglobulin G subclass 4; 274.RA.F4; RF mAb 61; mAb 61
Amount: 100 μg
Application Codes Clone: ELISA; HA
Shipping Temperature: Wet Ice
Origin Pub PMID: 17395205
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgM
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Human IgM format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for the C-terminal (Cγ3) end of the Fc region of IgG1, -2, and -3, with ~10-fold lower affinity for IgG4. This antibody's binding to IgG Fc is non-inhibitable by S. aureus protein A (SpA), which binds IgG Fc at the Cγ2–Cγ3 interface; this antibody's epitope is almost as far as physically possible from the antigen-combining site of IgG and is also remote from the functional sites responsible for FcγR or C1q binding (in Cγ2) and from the N-linked carbohydrate, also in Cγ2.
Application Notes: The binding activity of this antibody's original format (human IgM) to IgG Fc fragments was measured by ELISA, and a dissociation constant (K_d) of 6.0×10^-7 M was calculated (Burastero et al., 1988; PMID: 3264319). This antibody exhibited strong reactivity with both monoclonal and polyclonal human anti-Rh IgG antibodies bearing the Gm(a) or Gm(g) specificity in hemagglutination assays (HAs), displaying a higher affinity for Gm(g). Additionally, it demonstrated efficient binding to heat-altered autologous Gm(a-), (g-) IgG and exhibited heteroclitic specificity at the clonal level in an autologous system (Williams Jr et al., 1992; PMID: 1380541). The binding of this antibody's original format to human IgG subclasses as measured by ELISA demonstrated that its binding specificity is IgG1~IgG2~IgG3>IgG4 (Duquerroy et al., 2007; PMID: 17395205).

Product Class:	Purified
Clone ID:	RF61
Synonyms:	IgG1; IgG2; IgG3; IgG4; Immunoglobulin G; Immunoglobulin gamma-1; Immunoglobulin G subclass 1; Immunoglobulin gamma-2; Immunoglobulin G subclass 2; Immunoglobulin gamma-3; Immunoglobulin G subclass 3; Immunoglobulin gamma-4; Immunoglobulin G subclass 4; 274.RA.F4; RF mAb 61; mAb 61
Amount:	100 μg
Application Codes Clone:	ELISA; HA
Shipping Temperature:	Wet Ice
Origin Pub PMID:	17395205
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgM
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric mouse antibody was made using the variable domain sequences of the original Human IgM format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for the C-terminal (Cγ3) end of the Fc region of IgG1, -2, and -3, with ~10-fold lower affinity for IgG4. This antibody's binding to IgG Fc is non-inhibitable by S. aureus protein A (SpA), which binds IgG Fc at the Cγ2–Cγ3 interface; this antibody's epitope is almost as far as physically possible from the antigen-combining site of IgG and is also remote from the functional sites responsible for FcγR or C1q binding (in Cγ2) and from the N-linked carbohydrate, also in Cγ2.
Application Notes:	The binding activity of this antibody's original format (human IgM) to IgG Fc fragments was measured by ELISA, and a dissociation constant (K_d) of 6.0×10^-7 M was calculated (Burastero et al., 1988; PMID: 3264319). This antibody exhibited strong reactivity with both monoclonal and polyclonal human anti-Rh IgG antibodies bearing the Gm(a) or Gm(g) specificity in hemagglutination assays (HAs), displaying a higher affinity for Gm(g). Additionally, it demonstrated efficient binding to heat-altered autologous Gm(a-), (g-) IgG and exhibited heteroclitic specificity at the clonal level in an autologous system (Williams Jr et al., 1992; PMID: 1380541). The binding of this antibody's original format to human IgG subclasses as measured by ELISA demonstrated that its binding specificity is IgG1~IgG2~IgG3>IgG4 (Duquerroy et al., 2007; PMID: 17395205).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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